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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Glia. 2013 Dec 6;62(2):259–271. doi: 10.1002/glia.22604

Figure 3. Effect of ethanol on neurocan protein and mRNA levels in primary astrocytes.

Figure 3

A, B, D: Primary rat cortical astrocytes were incubated for 24 h in the presence or absence of 75 mM ethanol. Neurocan protein levels were quantified in cell lysates (A) and astrocyte-conditioned media (B) by ELISA. C: Representative immunoblots of neurocan forms in astrocyte cell lysate (left) and astrocyte-conditioned medium (right). D: Neurocan mRNA levels were quantified by qPCR using TaqMan probes and primers; results were normalized to β-actin and expressed as fold over control. **: p< 0.01; ***: p< 0.001 compared with control by Student’s t test (n=6).