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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Dec;70(12 Pt 1-2):3621–3625. doi: 10.1073/pnas.70.12.3621

Infectious DNA from Herpes Simplex Virus: Infectivity of Double-stranded and Single-stranded Molecules

P Sheldrick *, M Laithier *, D Lando , M L Ryhiner
PMCID: PMC427293  PMID: 4357884

Abstract

The infectious units in native and alkalidenatured preparations of DNA of herpes simplex virus were characterized with respect to their sensitivity to Neurospora crassa endonuclease, their sedimentation properties in high-salt, neutral sucrose gradients, and their sensitivity to hydrodynamic shearing forces. Infectious molecules in native preparations were resistant to N. crassa endonuclease, sedimented at 56 S, and were highly sensitive to shearing forces. After alkaline denaturation, infectious molecules became sensitive to the N. crassa enzyme, sedimented at 200 S, and were relatively resistant to shear. We conclude that both intact duplex molecules ([unk]100 × 106 daltons) and intact single strands ([unk]50 × 106 daltons) are capable of initiating productive infection.

Keywords: N. crassa endonuclease, sucrose gradient centrifugation, hydrodynamic shear

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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