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. 1973 Dec;70(12 Pt 1-2):3646–3649. doi: 10.1073/pnas.70.12.3646

Cell-Free Synthesis of Rat Growth Hormone

F Carter Bancroft 1, Guang-Jer Wu 1, Geoffrey Zubay 1
PMCID: PMC427298  PMID: 4519652

Abstract

Growth hormone has been synthesized in a cell-free system derived from Krebs II ascites cells, under the direction of RNA prepared from rat pituitary tumor (GC) cells. Growth hormone synthesized in the cell-free system was identified by precipitation with antiserum against growth hormone developed in baboon, followed by electrophoretic analysis of the dissolved precipitate on sodium dodecyl sulfate-polyacrylamide gels. RNA from both the membrane and the post-membrane fractions of the cytoplasm of GC cells stimulated protein synthesis in the cell-free system, but only RNA from the membrane fraction was found to direct the synthesis of growth hormone.

Keywords: pituitary tumor cells, Krebs II ascites cells, membrane

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Selected References

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