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. 1973 Dec;70(12 Pt 1-2):3679–3683. doi: 10.1073/pnas.70.12.3679

DNA Polymerase III Star Requires ATP to Start Synthesis on a Primed DNA

William Wickner 1, Arthur Kornberg 1
PMCID: PMC427305  PMID: 4519657

Abstract

DNA polymerase III star replicates a ϕX174 single-stranded, circular DNA primed with a fragment of RNA. This reaction proceeds in two stages. In stage I, a complex is formed requiring DNA polymerase III star, ATP, spermidine, copolymerase III*, and RNA-primed ϕX174 single-stranded, circular DNA. The complex, isolated by gel filtration, contains ADP and inorganic phosphate (the products of a specific ATP cleavage) as well as spermidine, polymerase III star, and copolymerase III star. In stage II, the chain grows upon addition of deoxynucleoside triphosphates; ADP and inorganic phosphate are discharged and chain elongation is resistant to antibody to copolymerase III star. Thus ATP and copolymerase III star are required to initiate chain growth but not to sustain it.

Keywords: spermidine-copolymerase III star-pol III* complex

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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