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. 1973 Dec;70(12 Pt 1-2):3744–3748. doi: 10.1073/pnas.70.12.3744

Size and Base Composition of RNA in Supercoiled Plasmid DNA

Peter H Williams *, Herbert W Boyer †, Donald R Helinski *
PMCID: PMC427319  PMID: 4359488

Abstract

The average size and base composition of the covalently integrated RNA segment in supercoiled ColE1 DNA synthesized in Escherichia coli in the presence of chloramphenicol (CM-ColE1 DNA) have been determined by two independent methods. The two approaches yielded similar results, indicating that the RNA segment in CM-ColE1 DNA contains GMP at the 5′ end and comprises on the average 25 to 26 ribonucleotides with a base composition of 10-11 G, 3 A, 5-6 C, and 6-7 U.

Keywords: Escherichia coli, RNase-sensitive DNA, DNA replication, colicinogenic factor E1, polymerase of Rous sarcoma virus

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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