Abstract
Veratridine, an activator of action potential Na+ ionophores, stimulated passive Na+ uptake by electrically excitable neuroblastoma and muscle cells but had no effect on clonal cell lines defective in Na+-ionophore activity. Veratridine-dependent Na+ uptake was completely inhibited by tetrodotoxin, a specific inhibitor of the action potential Na+ ionophore. Half-maximal inhibition was obtained with 11 nM tetrodotoxin. Thus, veratridinedependent Na+ uptake provides a specific and convenient means of assaying populations of cultured cells for action potential Na+-ionophore activity.
Keywords: excitable membranes, hybrid cells, veratridine, tetrodotoxin
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Albuquerque E. X., Daly J. W., Witkop B. Batrachotoxin: chemistry and pharmacology. Science. 1971 Jun 4;172(3987):995–1002. doi: 10.1126/science.172.3987.995. [DOI] [PubMed] [Google Scholar]
- Amano T., Richelson E., Nirenberg M. Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase-acetylcholinesterase-tyrosine hydroxylase-axons-dendrites). Proc Natl Acad Sci U S A. 1972 Jan;69(1):258–263. doi: 10.1073/pnas.69.1.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benzer T. I., Raftery M. A. Partial characterization of a tetrodotoxin-binding component from nerve membrane. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3634–3637. doi: 10.1073/pnas.69.12.3634. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Colquhoun D., Henderson R., Ritchie J. M. The binding of labelled tetrodotoxin to non-myelinated nerve fibres. J Physiol. 1972 Dec;227(1):95–126. doi: 10.1113/jphysiol.1972.sp010022. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans M. H. Tetrodotoxin, saxitoxin, and related substances: their applications in neurobiology. Int Rev Neurobiol. 1972;15:83–166. doi: 10.1016/s0074-7742(08)60329-3. [DOI] [PubMed] [Google Scholar]
- Fischbach G. D., Nameroff M., Nelson P. G. Electrical properties of chick skeletal muscle fibers developing in cell culture. J Cell Physiol. 1971 Oct;78(2):289–299. doi: 10.1002/jcp.1040780218. [DOI] [PubMed] [Google Scholar]
- HODGKIN A. L., HUXLEY A. F., KATZ B. Measurement of current-voltage relations in the membrane of the giant axon of Loligo. J Physiol. 1952 Apr;116(4):424–448. doi: 10.1113/jphysiol.1952.sp004716. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HODGKIN A. L., HUXLEY A. F. The dual effect of membrane potential on sodium conductance in the giant axon of Loligo. J Physiol. 1952 Apr;116(4):497–506. doi: 10.1113/jphysiol.1952.sp004719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harris A. J., Dennis M. J. Acetylcholine sensitivity and distribution on mouse neuroblastoma cells. Science. 1970 Feb 27;167(3922):1253–1255. doi: 10.1126/science.167.3922.1253. [DOI] [PubMed] [Google Scholar]
- KEYNES R. D. The ionic movements during nervous activity. J Physiol. 1951 Jun;114(1-2):119–150. doi: 10.1113/jphysiol.1951.sp004608. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kidokoro Y. Development of action potentials in a clonal rat skeletal muscle cell line. Nat New Biol. 1973 Jan 31;241(109):158–159. doi: 10.1038/newbio241158a0. [DOI] [PubMed] [Google Scholar]
- Konigsberg I. R. Diffusion-mediated control of myoblast fusion. Dev Biol. 1971 Sep;26(1):133–152. doi: 10.1016/0012-1606(71)90113-8. [DOI] [PubMed] [Google Scholar]
- Koppenhöfer E., Schmidt H. Incomplete sodium inactivation in nodes of Ranvier treated with scorpion venom. Experientia. 1968 Jan 15;24(1):41–42. doi: 10.1007/BF02136780. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Littlefield J. W. The use of drug-resistant markers to study the hybridization of mouse fibroblasts. Exp Cell Res. 1966 Jan;41(1):190–196. doi: 10.1016/0014-4827(66)90558-1. [DOI] [PubMed] [Google Scholar]
- Minna J., Glazer D., Nirenberg M. Genetic dissection of neural properties using somatic cell hybrids. Nat New Biol. 1972 Feb 23;235(60):225–231. doi: 10.1038/newbio235225a0. [DOI] [PubMed] [Google Scholar]
- Minna J., Nelson P., Peacock J., Glazer D., Nirenberg M. Genes for neuronal properties expressed in neuroblastoma x L cell hybrids. Proc Natl Acad Sci U S A. 1971 Jan;68(1):234–239. doi: 10.1073/pnas.68.1.234. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NARAHASHI T., MOORE J. W., SCOTT W. R. TETRODOTOXIN BLOCKAGE OF SODIUM CONDUCTANCE INCREASE IN LOBSTER GIANT AXONS. J Gen Physiol. 1964 May;47:965–974. doi: 10.1085/jgp.47.5.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Narahashi T., Moore J. W., Shapiro B. I. Condylactis toxin: interaction with nerve membrane ionic conductances. Science. 1969 Feb 14;163(3868):680–681. doi: 10.1126/science.163.3868.680. [DOI] [PubMed] [Google Scholar]
- Nelson P. G., Peacock J. H., Amano T., Minna J. Electrogenesis in mouse neuroblastoma cells in vitro. J Cell Physiol. 1971 Jun;77(3):337–352. doi: 10.1002/jcp.1040770308. [DOI] [PubMed] [Google Scholar]
- Nelson P. G., Peacock J. H., Amano T. Responses of neuroblastoma cells to iontophoretically applied acetylcholine. J Cell Physiol. 1971 Jun;77(3):353–362. doi: 10.1002/jcp.1040770309. [DOI] [PubMed] [Google Scholar]
- Nelson P., Ruffner W., Nirenberg M. Neuronal tumor cells with excitable membranes grown in vitro. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1004–1010. doi: 10.1073/pnas.64.3.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peper K., Trautwein W. The effect of aconitine on the membrane current in cardiac muscle. Pflugers Arch Gesamte Physiol Menschen Tiere. 1967;296(4):328–336. doi: 10.1007/BF00362532. [DOI] [PubMed] [Google Scholar]
- Repke K., Est M., Portius H. J. Uber die Ursache der Speciesunterschiede in der Digitalisempfindlichkeit. Biochem Pharmacol. 1965 Dec;14(12):1785–1802. doi: 10.1016/0006-2952(65)90269-8. [DOI] [PubMed] [Google Scholar]
- SKOU J. C. ENZYMATIC BASIS FOR ACTIVE TRANSPORT OF NA+ AND K+ ACROSS CELL MEMBRANE. Physiol Rev. 1965 Jul;45:596–617. doi: 10.1152/physrev.1965.45.3.596. [DOI] [PubMed] [Google Scholar]
- Seyama I., Narahashi T. Increase in sodium permeability of squid axon membranes by -dihydrograyanotoxin II. J Pharmacol Exp Ther. 1973 Feb;184(2):299–307. [PubMed] [Google Scholar]
- Ulbricht W. The effect of veratridine on excitable membranes of nerve and muscle. Ergeb Physiol. 1969;61:18–71. doi: 10.1007/BFb0111446. [DOI] [PubMed] [Google Scholar]
- Vogel Z., Sytkowski A. J., Nirenberg M. W. Acetylcholine receptors of muscle grown in vitro. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3180–3184. doi: 10.1073/pnas.69.11.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]