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. 1973 Dec;70(12 Pt 1-2):3815–3819. doi: 10.1073/pnas.70.12.3815

Effect of Interferon Treatment on Cellular Messenger RNA

Hilton B Levy 1, Freddie L Riley 1
PMCID: PMC427335  PMID: 4521208

Abstract

Cell monolayers wre treated with interferon, and then were labeled with RNA precursors. Messenger ribonucleoprotein and transfer RNA were liberated from isolated polysomes, and analyzed by electrophoresis on polyacrylamide gels. The messenger ribonucleoproteins and transfer RNA from interferon-treated cells migrated slightly slower than did the corresponding components from control cells. It was shown that the differences in migration speeds was attributable to the fact that the messenger RNAs and transfer RNA that were polysomebound (i.e., being translated) were slightly larger than control-cell RNAs. This phenomenon was seen in mouse L cells, primary mouse-embryo cells, and rabbit-kidney cells. Ribosomal 28S and 18S RNA was not altered, nor was nuclear RNA. Several types of evidence indicate that the biochemical changes are attributable to the interferon in the preparations, and not to impurities. These data may bear on the mechanism by which interferon-treated cells recognize and translate viral RNA poorly. It is tentatively suggested that interferon treatment of cells leads to a change in cellular messenger RNA such that the cell can distinguish it from the subsequently infecting viral RNA.

Keywords: messenger ribonucleoprotein, polyacrylamide electrophoresis, viral DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARON S., BUCKLER C. E. CIRCULATING INTERFERON IN MICE AFTER INTRAVENOUS INJECTION OF VIRUS. Science. 1963 Sep 13;141(3585):1061–1063. doi: 10.1126/science.141.3585.1061. [DOI] [PubMed] [Google Scholar]
  2. Carter W. A., Levy H. B. The recognition of viral RNA by mammalian ribosomes. An effect of interferon. Biochim Biophys Acta. 1968 Feb 26;155(2):437–443. doi: 10.1016/0005-2787(68)90189-5. [DOI] [PubMed] [Google Scholar]
  3. Friedman R. M., Metz D. H., Esteban R. M., Tovell D. R., Ball L. A., Kerr I. M. Mechanism of interferon action: inhibition of viral messenger ribonucleic acid translation in L-cell extracts. J Virol. 1972 Dec;10(6):1184–1198. doi: 10.1128/jvi.10.6.1184-1198.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Joklik W. K., Merigan T. C. Concerning the mechanism of action of interferon. Proc Natl Acad Sci U S A. 1966 Aug;56(2):558–565. doi: 10.1073/pnas.56.2.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kerr I. M., Sonnabendja, Martin E. M. Proein-synthetic activity of ribosomes from interferon-treated cells. J Virol. 1970 Feb;5(2):132–144. doi: 10.1128/jvi.5.2.132-144.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Levy H. B., Carter W. A. Molecular basis of the action of interferon. J Mol Biol. 1968 Feb 14;31(3):561–577. doi: 10.1016/0022-2836(68)90428-2. [DOI] [PubMed] [Google Scholar]
  7. Marcus P. I., Salb J. M. Molecular basis of interferon action: inhibition of viral RNA translation. Virology. 1966 Nov;30(3):502–516. doi: 10.1016/0042-6822(66)90126-7. [DOI] [PubMed] [Google Scholar]
  8. Oie H. K., Gazdar A. F., Buckler C. E., Baron S. High interferon producing line of transformed murine cells. J Gen Virol. 1972 Oct;17(1):107–109. doi: 10.1099/0022-1317-17-1-107. [DOI] [PubMed] [Google Scholar]
  9. Vilcek J., Ng M. H. Post-transcriptional control of interferon synthesis. J Virol. 1971 May;7(5):588–594. doi: 10.1128/jvi.7.5.588-594.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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