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. 1973 Dec;70(12 Pt 1-2):3825–3829. doi: 10.1073/pnas.70.12.3825

Isolation of cis-Zeatin from Corynebacterium fascians Cultures

Emanuel Scarbrough *, Donald J Armstrong *, Folke Skoog *, Charles R Frihart , Nelson J Leonard
PMCID: PMC427337  PMID: 16592126

Abstract

Three cytokinin-active constituents have been isolated from the culture medium of Corynebacterium fascians, a plant pathogen causing fasciation. One constituent is now identified as cis-zeatin [6-(4-hydroxy-3-methyl-cis-2-butenylamino)purine]. It represents the first unequivocal demonstration of the production of a zeatin isomer by a bacterial species. Another constituent was identified earlier as 6-(3-methyl-2-butenyl-amino)purine. The third constituent, distinct, on Sephadex LH-20 column chromatography, from the above two and their ribosides, has not yet been identified.

Keywords: cytokinins, 6-(4-hydroxy-3-methyl-cis-2-butenylamino)purine, 6-(3-methyl-2-butenylamino)purine, fasciation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D. J., Burrows W. J., Evans P. K., Skoog F. Isolation of cytokinins from tRNA. Biochem Biophys Res Commun. 1969 Oct 22;37(3):451–456. doi: 10.1016/0006-291x(69)90936-x. [DOI] [PubMed] [Google Scholar]
  2. Armstrong D. J., Skoog F., Kirkegaard L. H., Hampel A. E., Bock R. M., Gillam I., Tener G. M. Cytokinins: distribution in species of yeast transfer RNA. Proc Natl Acad Sci U S A. 1969 Jun;63(2):504–511. doi: 10.1073/pnas.63.2.504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hall R. H., Csonka L., David H., McLennan B. Cytokinins in the soluble RNA of plant tissues. Science. 1967 Apr 7;156(3771):69–71. doi: 10.1126/science.156.3771.69. [DOI] [PubMed] [Google Scholar]
  4. Helgeson J. P., Leonard N. J. Cytokinins: identification of compounds isolated from Corynebacterium fascians. Proc Natl Acad Sci U S A. 1966 Jul;56(1):60–63. doi: 10.1073/pnas.56.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klämbt D., Thies G., Skoog F. Isolation of cytokinins from Corynebacterium fascians. Proc Natl Acad Sci U S A. 1966 Jul;56(1):52–59. doi: 10.1073/pnas.56.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Leonard N. J., Hecht S. M., Skoog F., Schmitz R. Y. CYTOKININS: SYNTHESIS OF 6-(3-METHYL-3-BUTENYLAMINO)-9-beta-D-RIBOFURANOSYLPURINE (3IPA), AND THE EFFECT OF SIDE-CHAIN UNSATURATION ON THE BIOLOGICAL ACTIVITY OF ISOPENTYLAMINOPURINES AND THEIR RIBOSIDES. Proc Natl Acad Sci U S A. 1968 Jan;59(1):15–21. doi: 10.1073/pnas.59.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Matsubara S., Armstrong D. J., Skoog F. Cytokinins in tRNA of Corynebacterium fascians. Plant Physiol. 1968 Mar;43(3):451–453. doi: 10.1104/pp.43.3.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miller C. O. Zeatin and zeatin riboside from a mycorrhizal fungus. Science. 1967 Sep 1;157(3792):1055–1057. doi: 10.1126/science.157.3792.1055. [DOI] [PubMed] [Google Scholar]
  9. Phillips D. A., Torrey J. G. Studies on cytokinin production by Rhizobium. Plant Physiol. 1972 Jan;49(1):11–15. doi: 10.1104/pp.49.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Playtis A. J., Leonard N. J. The synthesis of ribosyl-cis-zeatin and thin layer chromatographic separation of the cis and trans isomers of ribosylzeatin. Biochem Biophys Res Commun. 1971 Oct 1;45(1):1–5. doi: 10.1016/0006-291x(71)90041-6. [DOI] [PubMed] [Google Scholar]
  11. Rogozinska J. H., Helgeson J. P., Skoog F. Partial Purification of a Cell-Division Factor from Peas. Plant Physiol. 1965 May;40(3):469–476. doi: 10.1104/pp.40.3.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schmitz R. Y., Skoog F. Cytokinins: synthesis and biological activity of geometric and position isomers of zeatin. Plant Physiol. 1972 Dec;50(6):702–705. doi: 10.1104/pp.50.6.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Upper C. D., Helgeson J. P., Kemp J. D., Schmidt C. J. Gas-Liquid Chromatographic Isolation of Cytokinins from Natural Sources: 6-(3-Methyl-2-butenylamino) purine from Agrobacterium Tumefaciens. Plant Physiol. 1970 May;45(5):543–547. doi: 10.1104/pp.45.5.543. [DOI] [PMC free article] [PubMed] [Google Scholar]

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