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. 1973 Dec;70(12 Pt 1-2):3843–3846. doi: 10.1073/pnas.70.12.3843

Effect of the Removal of the Y Base on the Conformation of Yeast tRNAPhe

David R Kearns 1, K Lim Wong 1,*, Yeng P Wong 1
PMCID: PMC427341  PMID: 4590172

Abstract

The effect of removing the Y base from the anticodon loop of yeast tRNAPhe has been examined by high-resolution proton nuclear magnetic resonance spectroscopy and optical melting. Analysis of the changes in the nuclear magnetic resonance spectra indicate that the removal of the Y base produces a small conformational change in the anticodon stem in which all the interbase separations are increased by approximately 0.2 Å. The temperature dependence of the nuclear magnetic resonance spectra and the optical melting measurements indicate that the high temperature stability of tRNAPhe is decreased by removal of the Y base. With the exception of the anticodon stem, the secondary structure of the other helical stems of the molecule appear to be unaltered when the Y base is excised.

Keywords: base pairing, 300-MHz proton NMR, conformation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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