Abstract
Lysates of an Escherichia coli polA- strain convert single-stranded DNA from ϕX174 virus to the double-stranded replicative form with high efficiency on cellophane discs. The initiation of synthesis of the complementary strand appears to be rate limiting; once initiation occurs, the chain is propagated rapidly. Under these conditions, the unsealed replicative form accumulates and is slowly converted to the sealed form; this conversion requires the activity of the DPN-dependent DNA ligase.
Keywords: ϕX174 DNA, DNA replication, E. coli polA-
Full text
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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