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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jan;69(1):25–29. doi: 10.1073/pnas.69.1.25

Replication of ϕX174 DNA by Escherichia coli polA-In Vitro

Baldomero M Olivera 1, Friedrich Bonhoeffer 1
PMCID: PMC427536  PMID: 4550507

Abstract

Lysates of an Escherichia coli polA- strain convert single-stranded DNA from ϕX174 virus to the double-stranded replicative form with high efficiency on cellophane discs. The initiation of synthesis of the complementary strand appears to be rate limiting; once initiation occurs, the chain is propagated rapidly. Under these conditions, the unsealed replicative form accumulates and is slowly converted to the sealed form; this conversion requires the activity of the DPN-dependent DNA ligase.

Keywords: ϕX174 DNA, DNA replication, E. coli polA-

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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