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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jan;69(1):34–36. doi: 10.1073/pnas.69.1.34

Cell Separation on Affinity Columns: The Isolation of Immunospecific Precursor Cells from Unimmunized Mice

Claudia Henry *, John Kimura *, Leon Wofsy *,
PMCID: PMC427538  PMID: 4500553

Abstract

We have used affinity columns to isolate from the spleens of unimmunized mice a population of lymphocytes that specifically bind a lactoside hapten. These cells are able to initiate an antibody response to azophenyl-β-lactoside when transferred to appropriate irradiated recipients.

Keywords: lactoside hapten, lymphocyte receptors, immunology

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ada G. L. Antigen binding cells in tolerance and immunity. Transplant Rev. 1970;5:105–129. doi: 10.1111/j.1600-065x.1970.tb00358.x. [DOI] [PubMed] [Google Scholar]
  2. Edelman G. M., Rutishauser U., Millette C. F. Cell fractionation and arrangement on fibers, beads, and surfaces. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2153–2157. doi: 10.1073/pnas.68.9.2153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hulett H. R., Bonner W. A., Barrett J., Herzenberg L. A. Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence. Science. 1969 Nov 7;166(3906):747–749. doi: 10.1126/science.166.3906.747. [DOI] [PubMed] [Google Scholar]
  4. Inman J. K., Dintzis H. M. The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents. Biochemistry. 1969 Oct;8(10):4074–4082. doi: 10.1021/bi00838a026. [DOI] [PubMed] [Google Scholar]
  5. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Wigzell H. Specific fractionation of immunocompetent cells. Transplant Rev. 1970;5:76–104. doi: 10.1111/j.1600-065x.1970.tb00357.x. [DOI] [PubMed] [Google Scholar]
  7. Wofsy L., Kimura J., Truffa-Bachi P. Cell separation on affinity columns: the preparation of pure populations of anti-hapten specific lymphocytes. J Immunol. 1971 Sep;107(3):725–729. [PubMed] [Google Scholar]

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