Abstract
The human complement component, Clq, is a fragile molecule of delicate structure consisting of three distinct parts, a central subunit, connecting strands, and terminal subunits. Each terminal subunit is further subdivided into a large and a small subunit, and the central subunit appears to be divided into two equal parts. In the intact molecule, six connecting strands link six terminal subunits by their larger subdivision to the central subunit. The overall diameter of the molecule when viewed from the “top” is about 35 nm (350 Å).
Keywords: negative staining, glutamine synthetase, biologically active Clq
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- Chesebro B., Bloth B., Svehag S. E. The ultrastructure of normal and pathological IgM immunoglobulins. J Exp Med. 1968 Mar 1;127(3):399–410. doi: 10.1084/jem.127.3.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Müller-Eberhard H. J. Chemistry and reaction mechanisms of complement. Adv Immunol. 1968;8:1–80. doi: 10.1016/s0065-2776(08)60464-2. [DOI] [PubMed] [Google Scholar]
- Shelton E., McIntire K. R. Ultrastructure of the gamma M immunoglobulin-molecule. J Mol Biol. 1970 Feb 14;47(3):595–597. doi: 10.1016/0022-2836(70)90327-x. [DOI] [PubMed] [Google Scholar]
- Svehag S. E., Bloth B. The ultrastructure of human C1q. Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(2):260–262. doi: 10.1111/j.1699-0463.1970.tb04299.x. [DOI] [PubMed] [Google Scholar]
- Valentine R. C., Shapiro B. M., Stadtman E. R. Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. Biochemistry. 1968 Jun;7(6):2143–2152. doi: 10.1021/bi00846a017. [DOI] [PubMed] [Google Scholar]
- Yonemasu K., Stroud R. M. Clq: rapid purification method for preparation of monospecific antisera and for biochemical studies. J Immunol. 1971 Feb;106(2):304–313. [PubMed] [Google Scholar]
- Yonemasu K., Stroud R. M., Niedermeier W., Butler W. T. Chemical studies of Clq; a modulator of immunoglobulin biology. Biochem Biophys Res Commun. 1971 Jun 18;43(6):1388–1394. doi: 10.1016/s0006-291x(71)80028-1. [DOI] [PubMed] [Google Scholar]