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. 1972 Jan;69(1):248–252. doi: 10.1073/pnas.69.1.248

Defective Formation of the Lamellar Cytoplasm by Neoplastic Fibroblasts

L V Domnina 1, O Y Ivanova 1, L B Margolis 1, L V Olshevskaja *, Y A Rovensky *, J M Vasiliev *, I M Gelfand 1
PMCID: PMC427585  PMID: 4500551

Abstract

Isolated cultures of mouse L-cells are similar to those of normal cells in showing contact inhibition of movement and topoinhibition of growth. In mixed cultures with untransformed mouse embryo fibroblasts, their parent strain, however, L cells are able to form colonies above the monolayer of normal fibroblasts, i.e., they have a property characteristic of transformed cells. Analysis of microcinematographic data suggests that the behavior of L cells in mixed cultures is a result of their defective attachment to the substratum. Scanning electron microscopy showed that attachment of a normal fibroblast was accompanied by the formation of a wide ring of flattened cytoplasm spread on the substratum (lamellar cytoplasm). This structure was observed to disintegrate in newly attached L cells. The structure of the lamellar cytoplasm remained abnormal in the fully spread L cells. The mean area of lamellar cytoplasm was 3- to 4-times less in L cells than in normal fibroblasts.

It is suggested that deficient formation of lamellar cytoplasm may be the basis of the inability of L cells to interact normally with embryo fibroblasts.

Keywords: L cells, transformed cells, cell attachment, contact inhibition, scanning electron microscopy, microcinematography

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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