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. 1972 Jan;69(1):278–282. doi: 10.1073/pnas.69.1.278

Primary Structure of the Ovine Hypothalamic Luteinizing Hormone-Releasing Factor (LRF)

Roger Burgus *, Madalyn Butcher *, Max Amoss *, Nicholas Ling *, Michael Monahan *, Jean Rivier *, Robert Fellows *, Richard Blackwell *, Wylie Vale *, Roger Guillemin *
PMCID: PMC427591  PMID: 4550508

Abstract

The primary structure of ovine hypothalamic hypophysiotropic luteinizing hormone-releasing factor, LRF, has been established as pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 by hydrolysis of the peptide with chymotrypsin or pyrrolidone-carboxylylpeptidase and by analysis of the products by an Edman-dansylation sequencing technique, as well as by mass spectrometry of the derived phenylthiohydantoins. A decapeptide with the proposed primary structure, prepared by total synthesis, gave the same result on sequencing. The synthetic decapeptide possesses the same biological activities as the native ovine LRF. The amino-acid sequence of ovine LRF is identical to that already published for porcine LRF.

Keywords: LH, hypothalamus, LRF, gas chromatography-mass spectrometry, decapeptide, Edman degradation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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