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. 1972 Jan;69(1):109–114. doi: 10.1073/pnas.69.1.109

Mutation Causing Temperature-Sensitive Expression of Cell Transformation by a Tumor Virus

Hartmut C Renger 1, Claudio Basilico 1
PMCID: PMC427597  PMID: 4109594

Abstract

A procedure has been devised to isolate 3T3 mouse fibroblasts transformed by simian virus 40 (SV40) that express their transformed phenotype at low (32°C) but not at high (39°C) temperature. Three parameters typical of malignant growth in vitro: (a) high saturation density in culture, (b) ability to form colonies on monolayers of normal 3T3 cells, and (c) lack of contact inhibition of DNA synthesis, are temperature sensitive. These phenotypic changes are fully reversible. The serum requirement for growth appears to be largely unchanged by temperature. These cells seem to owe their behavior to a cellular, rather than to a viral, alteration since after fusion of the temperature-sensitive transformed cells with permissive monkey cells, a procedure that leads to rescue (i.e., multiplication of the virus), wild-type SV40 virus is produced.

Keywords: SV40, 3T3 mouse cells, growth control

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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