Skip to main content
. 2014 Dec 8;6(12):5667–5678. doi: 10.3390/nu6125667

Figure 6.

Figure 6

Nuclear factor-erythroid 2 related factor 2 (Nrf2)-mediated induction and heme oxygenase-1 (HO-1) expression by Schisandrae Fructus ethanol extract in C2C12 cells. Cells were transfected with control small interfering RNA (siRNA) and Nrf2 siRNA. After 24 h, the cells were treated with 500 μg/mL Schisandrae Fructus ethanol extract (SFEE) for 6 h (A) or pretreated with 500 μg/mL SFEE. for 1 h and then stimulated with or without 1 mM H2O2 for 6 h (B). (A) Cellular proteins were separated on SDS-polyacrylamide gels and transferred to nitrocellulose membranes. The membranes were probed with specific antibodies against Nrf2 and HO-1. (B) Cell viability was estimated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results are mean ± SD values obtained from three independent experiments (* p < 0.05 compared with control group; # p < 0.05 compared with H2O2-treated group; $ p < 0.05 compared with H2O2 and SFEE-treated group).