Table 1. Forward and reverse primer sequences for RT-qPCR analysis of mRNA expression.
Gene | Forward primer 5′−3′ | Reverse primer 5′−3′ |
---|---|---|
ACAN | TGCATTCCACGAAGCTAACCT | GACGCCTCGCCTTCTTGA |
ACTA2 | GGAATGGGACAAAAAGACAGCTA | CGGGTACTTCAGGGTCAGGAT |
ASPN | TCACTTTATGGTCTGATCCTGAACA | CTTCGCAACTTCTTTGTGGTTAGA |
BGN | ACAAACTGCCCAGGAGTGAGTAG | GCCGTCCGCACGTCTATCT |
COL1A1 | CAACCTCAAGAAGTCCCTGC | AGGTGAATCGACTGTTGCCT |
DCN | TCAATGGACTGAACCAGATGA | CCTTGAGGAATGCTGGTGAT |
GAPDH | TGCACCACCAACTGCTTAGC | GGCATGGACTGTGGTCATGAG |
FMOD | CAGTATGAAGATGACCCTCATTGG | TAAGGGTCATAGGGATCGTAGTAGGT |
LUM | CTTCAATCAGATAGCCAGACTGC | AGCCAGTTCGTTGTGAGATAAAC |
TBP | CACGAACCACGGCACTGATT | TTTTCTTGCTGCCAGTCTGGAC |
VCAN | CAAGCATCCTGTCTCACGAA | CAACGGAAGTCATGCTCAAA |
YWHAZ | ACTTTTGGTACATTGTGGCTTCAA | CCGCCAGGACAAACCAGTAT |