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PikAIII sample preparation and raw EM images. a, SDS-PAGE gel of each purified form of PikAIII examined by cryo-EM. The numbers on the left indicate molecular weight in kDa. b, Raw EM image of holo-PikAIII particles embedded in negative stain. c, Raw cryo-EM image of holo-PikAIII particles. d, Boxed-out particle projections of holo-PikAIII.
