Figure 5.

Effect of chemical chaperones on early folding events. (A) SDS/PAGE gels obtained from E. coli lysates expressing frataxin. For each protein variant, the soluble (S) and insoluble (P) fractions are shown; (B) Semi-quantitative analysis of the relative proportion of frataxin present in the soluble and insoluble fractions, obtained from densitometric analysis of gel bands (n = 3), allowed the determination of the protein expressed in the soluble form; (C) Variation of the folding efficiency induced by the presence of chemical chaperones (increasing concentrations of TMAO and glycerol). After the densitometric analysis of the gel bands, the ratio between the amount of soluble protein present in the presence and absence of the compounds were determined. The Log2 of this ratio is here shown to highlight the variation observed.