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. 2014 Sep 25;5(21):10571–10583. doi: 10.18632/oncotarget.2512

Figure 6. Interaction of SOX10 with β-catenin and its DNA binding ability are required for tumor suppression.

Figure 6

(A) The schematic shows that the SOX10 contains a candidate β-catenin-binding region which is highly conserved. Below the schematic the ‘DB’, ‘3G’ and ‘482ins6′ mutations generated in SOX10 are shown. (B) IP assays showing that endogenous β-catenin binds exogenous V5-SOX10 and 482ins6 mutants, but not DB or 3G mutants. (C) HCT116 cells were cotransfected with V5-SOX10 or mutants plus the TOPFlash or c-Myc promoter reporter. Values are mean ± SD for triplicate samples from a representative experiment. *: p<0.05, **: p<0.01. (D) qRT-PCR and western blot showed ectopic expression of GFP, SOX10 and mutants on Wnt/β-catenin signaling pathway. (E) Schematic structure of the c-Myc promoter. TCF Binding Element (TBE) are indicated (Upper). ChIP assays on Wnt-responsive elements of c-Myc promoter gene were performed in HCT116 cells that were transfected with GFP, V5-SOX10 and mutants (lower). (F) Migration assay testing the ability of HCT116 cells expressing GFP, SOX10 and mutants to migrate through transwell chambers.