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. 2014 Sep 26;5(21):10678–10691. doi: 10.18632/oncotarget.2528

Figure 2. Knockdown of p53 blocks VMY-induced cell death.

Figure 2

LNCaP cells were infected with control or p53-shRNA adenovirus and incubated for 72 hrs followed by treatment with either DMSO or 30 uM VMY for 18hrs. (A) An approximate 50% reduction in p53 protein levels was observed (see inset, images are from the same autoradiogram and at the same exposure time). Cell death was significantly reduced in the p53shRNA treated cells as measured by trypan blue dye exclusion (*, p <0.01, N=3). (B) The effect of p53shRNA on the apoptotic machinery was confirmed by western blotting for p53 induction and for both full-length PARP (PARP-FL) and the long form of cleaved PARP (PARP-L, arrow).