Fig. 4. SRSF3 protects against CCl₄ toxicity.

(A) Gross morphology of 6 mo WT and HKO livers treated with CCl₄ for 4 weeks. White arrows indicate pre-cancerous nodules in HKO liver. (B) Liver sections from WT mice treated with oil or CCl₄ and stained for SRSF3. (C) Sirius Red staining for fibrosis (red) in oil and CCl₄-treated mice. (D) H&E and αSMA stained liver sections from oil- or CCl₄-treated mice showing steatosis (white arrows), portal inflammation (yellow arrows and inset) and myofibroblast activation (brown). (E) Mean fibrosis and inflammation scores from evaluation of multiple liver sections. (F) H&E and Sirius Red stained sections through a precancerous nodule in CCl₄-treated HKO liver. Nodule is outlined in black and labeled T.