Table 2.

Significant Proteins Identified on 2-DE and its Comparison with iTRAQ Data

Trend	Name of the protein	M.wt	2-DE	iTRAQ	Biological function	Molecular component
D-1	Succinyl-CoA synthetase beta chain	41.34	−5.79	−2.59	TCA	Ligase
D-2	Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit beta	36.69	−3.67	−4.31	Acetoin catabolism	Oxidoreductase
D-3	Elongation factor Tu	43.56	−2.40	−1.79	Protein biosynthesis	Elongation factor
D-4	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1	46.67	−2.24	−1.06	Cell division, cell wall biogenesis	Transferase
D-5	Succinyl-CoA ligase [ADP-forming] subunit beta	41.34	−2.06	−2.59	TCA	Ligase
D-6	Probable butyrate kinase	39.73	−2.05	−5.41	NA	Kinase
D-7	Ribosome-recycling factor	20.62	−1.81	−1.01	Protein biosynthesis	NA
D-8	Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex	47.37	−1.69	−1.04	Glycolysis	Transferase
D-9	Manganese-dependent inorganic pyrophosphatase	33.96	−1.57	−1.08	NA	Hydrolase
D-10	Phosphoenolpyruvate-protein phosphotransferase	63.03	−1.48	−1.41	Transport	Kinase, Transferase
D-11	3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 2	35.40	−1.46	−1.58	Lipid biosynthesis, fatty acid biosynthesis	Transferase
D-12	D-3-phosphoglycerate dehydrogenase	57.07	−1.41	−1.72	Serine biosynthesis	Oxidoreductase
D-13	Transcriptional regulatory protein degU	25.85	−1.13	−1.59	Transcription, two component system	Activator/repressor
U-1	Isoleucyl-tRNA synthetase	104.7	1.78	1.27	Protein synthesis	Ligase
U-2	Uncharacterized protein yvyD	21.96	2.11	1.70	Response	NA
U-3	Glyceraldehyde-3-phosphate dehydrogenase 1	35.67	2.14	2.31	Glycolysis	Oxidoreductase
U-4	ATP-dependent Clp protease ATP-binding subunit ClpX	46.37	2.23	−1.09	Stress response	Chaperone
U-5	NADPH dehydrogenase	55.12	2.63	3.56	Detoxification, Stress response	Oxidoreductase

NA=not available.