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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Nature. 2014 Oct 12;517(7532):104–107. doi: 10.1038/nature13797

Extended Data Fig. 3.

Extended Data Fig. 3

Yeast complementation assay. maSR1 can rescue the growth of a Saccharomyces cerevisiae Delta-14 sterol reductase ERG24 (yeast maSR1 homolog) deletion strain (ΔERG24). ΔERG24 yeast expressing wild-type maSR1, scERG24 and mutated maSR1 from a URA3 shuttle vector can grow under URA− selection (upper panel). Growth of yeast expressing maSR1, scERG24 and various mutated maSR1 versions under in the presence of sub-inhibitory concentrations of cycloheximide (20ng/ml) for 24 to 48 hours (lower panel). The yeast expressing maSR1 or scERG24 is able to grow in the presence of cycloheximide. R395A (lane 8) corresponds to R583Q in LBR which has been reported to lead to loss of activity in yeast35. Results are representative of three independent experiments.