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Solution scattering data. (a) A comparison of the primary sequence of hinge regions in the three IgG subclasses. The locations of the small rigid bodies are highlighted within the small blue box. (b) Scattering intensity plot and (c) Kratky plot of SAXS data collected on the IgGs in 50 mM Na phosphate, 100 mM NaCl, pH 7.4. IgG1 (blue), IgG2 (red) and IgG4 (green). (d) The pair distance distribution functions from the indirect Fourier transformation of the scattering intensity.
