Abstract
The Hoxd-11 gene was disrupted by homologous recombination in embryonic stem cells. We found that Hoxd-11-/- mutant mice are viable and display homeotic transformations of their sacral vertebrae, while their forelimbs present abnormalities of some metacarpals and of the first row of carpal bones. These results are discussed in the light of current models of tetrapod axial skeleton and limb patterning.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Davis A. P., Capecchi M. R. Axial homeosis and appendicular skeleton defects in mice with a targeted disruption of hoxd-11. Development. 1994 Aug;120(8):2187–2198. doi: 10.1242/dev.120.8.2187. [DOI] [PubMed] [Google Scholar]
- Dollé P., Dierich A., LeMeur M., Schimmang T., Schuhbaur B., Chambon P., Duboule D. Disruption of the Hoxd-13 gene induces localized heterochrony leading to mice with neotenic limbs. Cell. 1993 Nov 5;75(3):431–441. doi: 10.1016/0092-8674(93)90378-4. [DOI] [PubMed] [Google Scholar]
- Dollé P., Izpisúa-Belmonte J. C., Brown J. M., Tickle C., Duboule D. HOX-4 genes and the morphogenesis of mammalian genitalia. Genes Dev. 1991 Oct;5(10):1767–1767. doi: 10.1101/gad.5.10.1767. [DOI] [PubMed] [Google Scholar]
- Dollé P., Izpisúa-Belmonte J. C., Falkenstein H., Renucci A., Duboule D. Coordinate expression of the murine Hox-5 complex homoeobox-containing genes during limb pattern formation. Nature. 1989 Dec 14;342(6251):767–772. doi: 10.1038/342767a0. [DOI] [PubMed] [Google Scholar]
- Dollé P., Lufkin T., Krumlauf R., Mark M., Duboule D., Chambon P. Local alterations of Krox-20 and Hox gene expression in the hindbrain suggest lack of rhombomeres 4 and 5 in homozygote null Hoxa-1 (Hox-1.6) mutant embryos. Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7666–7670. doi: 10.1073/pnas.90.16.7666. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duboule D. The vertebrate limb: a model system to study the Hox/HOM gene network during development and evolution. Bioessays. 1992 Jun;14(6):375–384. doi: 10.1002/bies.950140606. [DOI] [PubMed] [Google Scholar]
- Haack H., Gruss P. The establishment of murine Hox-1 expression domains during patterning of the limb. Dev Biol. 1993 Jun;157(2):410–422. doi: 10.1006/dbio.1993.1145. [DOI] [PubMed] [Google Scholar]
- Izpisúa-Belmonte J. C., Falkenstein H., Dollé P., Renucci A., Duboule D. Murine genes related to the Drosophila AbdB homeotic genes are sequentially expressed during development of the posterior part of the body. EMBO J. 1991 Aug;10(8):2279–2289. doi: 10.1002/j.1460-2075.1991.tb07764.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krumlauf R. Hox genes in vertebrate development. Cell. 1994 Jul 29;78(2):191–201. doi: 10.1016/0092-8674(94)90290-9. [DOI] [PubMed] [Google Scholar]
- Lohnes D., Mark M., Mendelsohn C., Dollé P., Dierich A., Gorry P., Gansmuller A., Chambon P. Function of the retinoic acid receptors (RARs) during development (I). Craniofacial and skeletal abnormalities in RAR double mutants. Development. 1994 Oct;120(10):2723–2748. doi: 10.1242/dev.120.10.2723. [DOI] [PubMed] [Google Scholar]
- Lufkin T., Dierich A., LeMeur M., Mark M., Chambon P. Disruption of the Hox-1.6 homeobox gene results in defects in a region corresponding to its rostral domain of expression. Cell. 1991 Sep 20;66(6):1105–1119. doi: 10.1016/0092-8674(91)90034-v. [DOI] [PubMed] [Google Scholar]
- Milaire J. Etude morphologique, histochimique et autoradiographique de développement du squelette des membres chez l'embryon de souris. I. Membres antérieurs. Arch Biol (Liege) 1978;89(2):169–216. [PubMed] [Google Scholar]
- Morgan B. A., Izpisúa-Belmonte J. C., Duboule D., Tabin C. J. Targeted misexpression of Hox-4.6 in the avian limb bud causes apparent homeotic transformations. Nature. 1992 Jul 16;358(6383):236–239. doi: 10.1038/358236a0. [DOI] [PubMed] [Google Scholar]
- Small K. M., Potter S. S. Homeotic transformations and limb defects in Hox A11 mutant mice. Genes Dev. 1993 Dec;7(12A):2318–2328. doi: 10.1101/gad.7.12a.2318. [DOI] [PubMed] [Google Scholar]
- Summerbell D., Lewis J. H., Wolpert L. Positional information in chick limb morphogenesis. Nature. 1973 Aug 24;244(5417):492–496. doi: 10.1038/244492a0. [DOI] [PubMed] [Google Scholar]