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. 1974 Mar;5(3):337–343. doi: 10.1128/aac.5.3.337

Thermal Inactivation as a Means of Inhibiting the Serum-Associated Deamination of 9-β-d-Arabinofuranosyladenine in Tissue Culture Media

Pauline M Schwartz 1,2,3, Charles Shipman Jr 1,2,3, Roger H Carlson 1,2,3, John C Drach 1,2,3
PMCID: PMC428970  PMID: 4840442

Abstract

9-β-d-Arabinofuranosyladenine (ara-A) was deaminated to 9-β-d-arabinofuranosylhypoxanthine by adenosine deaminase present in fetal bovine serum, newborn calf serum, and calf serum used to supplement tissue culture media. Heating newborn calf serum or calf serum for 12 h at 56 C completely eliminated the enzymatic deamination of ara-A. The deaminase activity associated with fetal bovine serum was more refractory to heating, requiring 24 h for complete inactivation. The nutritive value of heat-inactivated calf serum did not differ significantly from that of unheated serum based on considerations of population doubling times, deoxyribonucleic acid synthesis, and relative cloning efficiencies of KB cells.

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Selected References

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