Abstract
An agar diffusion method using paper disks on plates seeded with Clostridium perfringens and incubated anaerobically to assay serum levels of metronidazole is described. The lowest serum metronidazole concentration that could be measured was 0.7 μg/ml, and the average error was 4% for 5 μg/ml. This assay could measure metronidazole in the presence of gentamicin or in the presence of penicillin, with the use of penicillinase incorporated into the assay plates, or in presence of penicillin, methicillin, and cephalothin with prior exposure of the specimen to beta-lactamase.
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- KANE P. O., McFADZEAN J. A., SQUIRES S., KING A. J., NICOL C. S. Absorption and excretion of metronidazole. I. Serum concentration and urinary excretion after oral administration. Br J Vener Dis. 1961 Dec;37:273–275. doi: 10.1136/sti.37.4.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sabath L. D., Casey J. I., Ruch P. A., Stumpf L. L., Finland M. Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma. J Lab Clin Med. 1971 Sep;78(3):457–463. [PubMed] [Google Scholar]
- Tally F. P., Sutter V. L., Finegold S. M. Metronidazole versus anaerobes. In vitro data and initial clinical observations. Calif Med. 1972 Dec;117(6):22–26. [PMC free article] [PubMed] [Google Scholar]