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. 2014 Oct 29;40(3):766–773. doi: 10.1038/npp.2014.256

Figure 1.

Figure 1

GFP-Gαs recovery after photobleaching is slower after chronic but not acute antidepressant treatment. C6 glioma cells stably expressing GFP-Gαs were cultured in phenol-red-free DMEM and membrane regions were photobleached. (a) Demonstration of representative photobleaching and recovery of GFP-Gαs. Scale bar represents 10 mm. (b) Demonstration of time course of recovery after photobleaching of control and 10 μM escitalopram or R-citalopram (72 h) -treated cells. Half-time to recovery of GFP-Gαs is increased after (c) chronic (72 h) but not (d) acute (1 h) escitalopram, desipramine, and fluoxetine treatments at 10 μM. Chronic (72 h) R-citalopram had no effect on half-time of recovery. Data were analyzed by one-way ANOVA followed by Tukey's test for post hoc multiple comparisons of means (control vs treatment, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). Error bars represent SEM.