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. 1975 Jan;7(1):22–24. doi: 10.1128/aac.7.1.22

Effect of Isoniazid on the Protoplasmic Viscosity in Mycobacterium tuberculosis

Kuni Takayama *, Alec D Keith †, Wallace Snipes †
PMCID: PMC429065  PMID: 806256

Abstract

The effect of isoniazid on the protoplasmic viscosity in the H37Ra strain of Mycobacterium tuberculosis was determined by using electron spin resonance spectroscopy and a small spin label tempone (2,2,6,6-tetramethylpiperidone-N-oxyl radical). Isoniazid (0.5 μg/ml) caused the internal cellular viscosity to increase gradually over the first 15 h of exposure from a rotational correlation time value (Tc) of 2.4 × 10−10 to 3.4 × 1010 s and then decrease linearly to the control level after 27 h. These results could be interpreted to mean that isoniazid allows a continued and normal synthesis of the protoplasmic components while the rate of increase in the cell volume is reduced. A degradative process may begin after the initial 15-h exposure time, which would cause the reduction in the internal viscosity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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