Abstract
Cells of Saccharomyces cerevisiae showed differential growth inhibition when cultured on various carbon sources in the presence of the proline analogue thiazolidine-4-carboxylic acid (TZ). On 0.5% yeast extract, 2% glucose and TZ (10 mg/ml) medium, growth lags from 8 to 10 h were observed, after which cells recovered and growth proceeded normally. Growth was totally inhibited on a medium of 0.5% yeast extract, 3% ethanol, and 5 mg of TZ per ml. This inhibition was not due to the inability of cells to undergo aerobic respiration, since similar media containing glycerol instead of ethanol allowed growth. Proline added to the culture medium reversed the lag on glucose and TZ medium but did not promote recovery on ethanol and TZ medium. TZ was found to have two probable modes of action in yeast. It was a noncompetitive inhibitor of yeast alcohol dehydrogenase, and it was also found to be incorporated into cellular protein. Uptake studies using 14C-labeled TZ showed that the recovery on glucose was correlated with the progressive exclusion of the analogue from cells.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dahlbom R., Tolf B. R., Akeson A., Lundquist G., Theorell H. On the inhibitory power of some further pyrazole derivatives of horse liver alcohol dehydrogenase. Biochem Biophys Res Commun. 1974 Apr 8;57(3):549–553. doi: 10.1016/0006-291x(74)90581-6. [DOI] [PubMed] [Google Scholar]
- EATON N. R. New press for disruption of microorganisms. J Bacteriol. 1962 Jun;83:1359–1360. doi: 10.1128/jb.83.6.1359-1360.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuznar J., Schwencke J., Magaña-Schwencke N. Sarcosine and imino acid uptake in Saccharomyces chevalieri. Derepression by nitrogen starvation. Biochim Biophys Acta. 1973 Aug 22;318(2):273–280. doi: 10.1016/0005-2736(73)90120-x. [DOI] [PubMed] [Google Scholar]
- MACKENZIE C. G., HARRIS J. N-formylcysteine synthesis in mitochondria from formaldehyde and L-cysteine via thiazolidinecarboxylic acid. J Biol Chem. 1957 Jul;227(1):393–406. [PubMed] [Google Scholar]
- Magaña-Schwencke N., Schwencke J. A proline transport system in Saccharomyces chevalieri. Biochim Biophys Acta. 1969 Mar 11;173(2):313–323. doi: 10.1016/0005-2736(69)90114-x. [DOI] [PubMed] [Google Scholar]
- ODEN S., VON HOFSTEN B. Detection of fingerprints by the ninhydrin reaction. Nature. 1954 Mar 6;173(4401):449–450. doi: 10.1038/173449a0. [DOI] [PubMed] [Google Scholar]
- Reynier M. Pyrazole inhibition and kinetic studies of ethanol and retinol oxidation catalyzed by rat liver alcohol dehydrogenase. Acta Chem Scand. 1969;23(4):1119–1129. doi: 10.3891/acta.chem.scand.23-1119. [DOI] [PubMed] [Google Scholar]
- Schwencke J., Magaña-Schwencke N. Derepression of a proline transport system in Saccharomyces chevalieri by nitrogen starvation. Biochim Biophys Acta. 1969 Mar 11;173(2):302–312. doi: 10.1016/0005-2736(69)90113-8. [DOI] [PubMed] [Google Scholar]
- Unger L., DeMoss R. D. Action of a proline analogue, l-thiazolidine-4-carboxylic acid, in Escherichia coli. J Bacteriol. 1966 Apr;91(4):1556–1563. doi: 10.1128/jb.91.4.1556-1563.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unger L., DeMoss R. D. Metabolism of a proline analogue, l-thiazolidine-4-carboxylic acid, by Escherichia coli. J Bacteriol. 1966 Apr;91(4):1564–1569. doi: 10.1128/jb.91.4.1564-1569.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
- Wilkie D. Selective inhibition of mitochondrial synthesis in Saccharomyces cerevisiae by canavanine. J Mol Biol. 1970 Jan 14;47(1):107–113. doi: 10.1016/0022-2836(70)90406-7. [DOI] [PubMed] [Google Scholar]