FIG. 3.

Overexpression of transmembrane protease serine 4 (TMPRSS4) enhances the proliferation of thyroid cancer cells. (A) Overexpression of TMPRSS4 in thyroid cancer cell lines was analyzed by Western blotting. α-Tubulin was used as a loading control. (B) MTT assays were performed to evaluate the effect of TMPRSS4 overexpression on the proliferation of the studied thyroid cancer cells at the indicated time points. (C) Representative micrographs of colony formation assays of the studied cells. (D) Representative images of anchorage-independent colonies formed. (E) Flow-cytometric determination of proportion of studied cells in distinct cell-cycle phases. (F) Quantification of 5-ethynyl-2'-deoxyuridine (EdU) incorporation assays. For (B) and (F), the data are reported as mean±standard deviation of three independent experiments. *p<0.05.