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. 1975 Jan;7(1):107–109. doi: 10.1128/aac.7.1.107

Comparison of a Radioimmunoassay with an Enzymatic Assay for Gentamicin

Barbara H Minshew 1,1, Randall K Holmes 1, Charles R Baxter 1
PMCID: PMC429081  PMID: 1137354

Abstract

A radioimmunoassay and an enzymatic assay for gentamicin have been compared. The correlation coefficient for results of gentamicin assays performed by the two methods with 45 serum specimens was 0.90. A similar standard curve for the radioimmunoassay was obtained with gentamicin complex, with gentamicin Cl, Cla, or C2, or with sisomicin as ligand, but tobramycin did not compete with [3H]gentamicin for binding to the antiserum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alcid D. V., Seligman S. J. Simplified assay for gentamicin in the presence of other antibiotics. Antimicrob Agents Chemother. 1973 May;3(5):559–561. doi: 10.1128/aac.3.5.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ekins R. P. Radioimmunoassay and saturation analysis. Basic principles and theory. Br Med Bull. 1974 Jan;30(1):3–11. doi: 10.1093/oxfordjournals.bmb.a071163. [DOI] [PubMed] [Google Scholar]
  3. GOODFRIEND T. L., LEVINE L., FASMAN G. D. ANTIBODIES TO BRADYKININ AND ANGIOTENSIN: A USE OF CARBODIIMIDES IN IMMUNOLOGY. Science. 1964 Jun 12;144(3624):1344–1346. doi: 10.1126/science.144.3624.1344. [DOI] [PubMed] [Google Scholar]
  4. Haas M. J., Davies J. Enzymatic acetylation as a means of determining serum aminoglycoside concentrations. Antimicrob Agents Chemother. 1973 Oct;4(4):497–499. doi: 10.1128/aac.4.4.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Herbert V., Lau K. S., Gottlieb C. W., Bleicher S. J. Coated charcoal immunoassay of insulin. J Clin Endocrinol Metab. 1965 Oct;25(10):1375–1384. doi: 10.1210/jcem-25-10-1375. [DOI] [PubMed] [Google Scholar]
  6. Holmes R. K., Sanford J. P. Enzymatic assay for gentamicin and related aminoglycoside antibiotics. J Infect Dis. 1974 May;129(5):519–527. doi: 10.1093/infdis/129.5.519. [DOI] [PubMed] [Google Scholar]
  7. Lewis J. E., Nelson J. C., Elder H. A. Radioimmunoassay of an antibiotic: gentamicin. Nat New Biol. 1972 Oct 18;239(94):214–216. doi: 10.1038/newbio239214a0. [DOI] [PubMed] [Google Scholar]
  8. Lund M. E., Blazevic D. J., Matsen J. M. Rapid gentamicin bioassay using a multiple-antibiotic-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother. 1973 Nov;4(5):569–573. doi: 10.1128/aac.4.5.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mahon W. A., Ezer J., Wilson T. W. Radioimmunoassay for measurement of gentamicin in blood. Antimicrob Agents Chemother. 1973 May;3(5):585–589. doi: 10.1128/aac.3.5.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Noone P., Pattison J. R., Samson D. Simple, rapid method for assay of aminoglycoside antibiotics. Lancet. 1971 Jul 3;2(7714):16–19. doi: 10.1016/s0140-6736(71)90007-9. [DOI] [PubMed] [Google Scholar]
  11. Sabath L. D., Casey J. I., Ruch P. A., Stumpf L. L., Finland M. Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma. J Lab Clin Med. 1971 Sep;78(3):457–463. [PubMed] [Google Scholar]
  12. Smith A. L., Smith D. H. Gentamicin:adenine mononucleotide transferase: partial purification, characterization, and use in the clinical quantitation of gentamicin. J Infect Dis. 1974 Apr;129(4):391–401. doi: 10.1093/infdis/129.4.391. [DOI] [PubMed] [Google Scholar]
  13. Smith D. H., Van Otto B., Smith A. L. A rapid chemical assay for gentamicin. N Engl J Med. 1972 Mar 16;286(11):583–586. doi: 10.1056/NEJM197203162861106. [DOI] [PubMed] [Google Scholar]

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