Abstract
Factors affecting candicidin synthesis and mycelial growth of Streptomyces griseus IMRU 3570 were studied. Inorganic phosphate was found to inhibit candicidin synthesis but to stimulate mycelial growth. Zinc, iron, and magnesium ions stimulated candicidin synthesis at relatively high concentrations in a complex medium but not in a synthetic medium. No other factors studied, such as temperature, oxygen absorption rate, and sugar concentration, were found to differentially affect antibiotic synthesis and mycelial growth. Optimum concentration of inorganic phosphate for candicidin synthesis in a chemically defined medium was found to be between 5 × 10−5 and 5 × 10−4 M. The culture in idiophase stage can be reverted to typical trophophase growth by the addition of inorganic phosphate, suggesting the controlling role of inorganic phosphate in repression and derepression of secondary metabolic and primary metabolic activity of the culture. With a soya peptone-glucose medium, the maximum rate of candicidin production could be maintained and extended for a considerable length of time by controlling the culture pH at 8.0, using glucose to adjust the pH during the later stages of a batch fermentation. Carrying out fermentations in this way has given candicidin yields up to 4 g/liter.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ACKER R. F., LECHEVALIER H. Some nutritional requirements of Streptomyces griseus 3570 for growth and candicidin production. Appl Microbiol. 1954 May;2(3):152–157. doi: 10.1128/am.2.3.152-157.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lampen J. O. Amphotericin B and other polyenic antifungal antibiotics. Am J Clin Pathol. 1969 Aug;52(2):138–146. doi: 10.1093/ajcp/52.2.138. [DOI] [PubMed] [Google Scholar]
- Liu C. M., McDaniel L. E., Schaffner C. P. Fungimycin, biogenesis of its aromatic moiety. J Antibiot (Tokyo) 1972 Mar;25(3):187–188. doi: 10.7164/antibiotics.25.187. [DOI] [PubMed] [Google Scholar]
- Liu C. M., McDaniel L. E., Schaffner C. P. Studies on candicidin biogenesis. J Antibiot (Tokyo) 1972 Feb;25(2):116–121. doi: 10.7164/antibiotics.25.116. [DOI] [PubMed] [Google Scholar]
- McDaniel L. E., Bailey E. G. Liquid nitrogen preservation of standard inoculum: gas-phase storage. Appl Microbiol. 1968 Jun;16(6):912–916. doi: 10.1128/am.16.6.912-916.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller A. L., Walker J. B. Accumulation of streptomycin-phosphate in cultures of streptomycin producers grown on a high-phosphate medium. J Bacteriol. 1970 Oct;104(1):8–12. doi: 10.1128/jb.104.1.8-12.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PERLMAN D., WAGMAN G. H. Studies on the utilization of lipids by streptomyces griseus. J Bacteriol. 1952 Feb;63(2):253–262. doi: 10.1128/jb.63.2.253-262.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walker J. B. Enzymatic reactions involved in streptomycin biosynthesis and metabolism. Lloydia. 1971 Dec;34(4):363–371. [PubMed] [Google Scholar]
- Walker M. S., Walker J. B. Streptomycin biosynthesis and metabolism. Enzymatic phosphorylation of dihydrostreptobiosamine moieties of dihydro-streptomycin-(streptidino) phosphate and dihydrostreptomycin by Streptomyces extracts. J Biol Chem. 1970 Dec 25;245(24):6683–6689. [PubMed] [Google Scholar]