Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1975 Feb;7(2):203–207. doi: 10.1128/aac.7.2.203

Effects of Adenine Arabinoside on Cellular Immune Mechanisms in Humans

Russell W Steele *, Isidoro A Chapa †,‡, Monroe M Vincent †,‡, Sally A Hensen †,‡, Ronald E Keeney †,‡
PMCID: PMC429104  PMID: 1137371

Abstract

In vitro lymphocyte blastogenic responses to the commonly employed mitogens, phytohemagglutinin, pokeweed, and concanavalin A, were evaluated when adenine arabinoside (ara-A) in a concentration of 3 μg/ml was added to the culture materials. Similarly, blastogenic and cytotoxic responses to cell cultures persistently infected with herpes simplex virus 1, herpes simplex virus 2, and varicella-zoster virus were determined in the presence of ara-A. No depression of these cellular immune responses by ara-A was demonstrated. This was in contrast to the effect of cytosine arabinoside, which at a concentration of 3 μg/ml severely inhibited these immune responses. Further studies examined lymphocyte blastogenic responses to the mitogens and blastogenic and cytotoxic responses specific for the herpes group virus infecting patients who were subsequently treated with ara-A; determinations were made before, during, and after treatment. In vitro responses during and after treatment with ara-A were unchanged or often enhanced as compared to pretreatment values. Therefore, the antiviral chemotherapeutic agent, ara-A, does not appear to depress the host's cellular immune responses, which are vital to successful elimination of invading herpes group viruses.

Full text

PDF
203

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buskirk H. H., Crim J. A., Petering H. G., Merritt K., Johnson A. G. Effect of uracil mustard and several antitumor drugs on the primary antibody response in rats and mice. J Natl Cancer Inst. 1965 Jun;34(6):747–758. [PubMed] [Google Scholar]
  2. Ch'ien L. T., Cannon N. J., Charamella L. J., Dismukes W. E., Whitley R. J., Buchanan R. A., Alford C. A., Jr Effect of adenine arabinoside on severe Herpesvirus hominis infections in man. J Infect Dis. 1973 Nov;128(5):658–663. doi: 10.1093/infdis/128.5.658. [DOI] [PubMed] [Google Scholar]
  3. Gray G. D., Nichol F. R., Mickelson M. M., Camiener G. W., Gish D. T., Kelly R. C., Wechter W. J., Moxley T. E., Neil G. L. Immunosuppressive, antiviral and antitumor activities of cytarabine derivatives. Biochem Pharmacol. 1972 Feb 15;21(4):465–475. doi: 10.1016/0006-2952(72)90319-x. [DOI] [PubMed] [Google Scholar]
  4. Kurtz S. M., Fisken R. A., Kaump D. H., Schardn J. L. Toxicity of 9-beta-D-arabinofuranosyladenine in mice and rabbits. Antimicrob Agents Chemother (Bethesda) 1968;8:180–189. [PubMed] [Google Scholar]
  5. Percy D. H., Albert D. M. Developmental defects in rats treated postnatally with 5-iododeoxyuridine (IUDR). Teratology. 1974 Jun;9(3):275–286. doi: 10.1002/tera.1420090307. [DOI] [PubMed] [Google Scholar]
  6. Silk B. R., Roome A. P. Herpes encephalitis treated with intravenous idoxuridine. Lancet. 1970 Feb 21;1(7643):411–412. doi: 10.1016/s0140-6736(70)91536-9. [DOI] [PubMed] [Google Scholar]
  7. Sloan B. J., Miller F. A., McLean I. W., Jr Treatment of herpes simplex virus types 1 and 2 encephalitis in mice with 9-beta-D-arabinofuranosyladenine. Antimicrob Agents Chemother. 1973 Jan;3(1):74–80. doi: 10.1128/aac.3.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Steele R. W., Hensen S. A., Vincent M. M., Fuccillo D. A., Bellanti J. A. A 51 Cr microassay technique for cell-mediated immunity to viruses. J Immunol. 1973 Jun;110(6):1502–1510. [PubMed] [Google Scholar]
  9. Steele R. W., Limas C., Thurman G. B., Schuelein M., Bauer H., Bellanti J. A. Familial thymic aplasia. Attempted reconstitution with fetal thymus in a Millipore diffusion chamber. N Engl J Med. 1972 Oct 19;287(16):787–791. doi: 10.1056/NEJM197210192871602. [DOI] [PubMed] [Google Scholar]
  10. Stevens D. A., Jordan G. W., Waddell T. F., Merigan T. C. Adverse effect of cytosine arabinoside on disseminated zoster in a controlled trial. N Engl J Med. 1973 Oct 25;289(17):873–878. doi: 10.1056/NEJM197310252891701. [DOI] [PubMed] [Google Scholar]
  11. Thong Y. H., Steele R. W., Vincent M. M., Hensen S. A., Bellanti J. A. Impaired in vitro cell-mediated immunity to rubella virus during pregnancy. N Engl J Med. 1973 Sep 20;289(12):604–606. doi: 10.1056/NEJM197309202891203. [DOI] [PubMed] [Google Scholar]
  12. Thorsby E. Cell specific and common antigens on human granulocytes and lymphocytes demonstrated with cytotoxic hetero-antibodies. Vox Sang. 1967 Aug;13(2):194–206. doi: 10.1111/j.1423-0410.1967.tb03949.x. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES