Abstract
To reduce the incubation time requirement in the Bauer-Kirby antibiotic susceptibility test, comparisons were made of the test results at 18 to 20 h (standard) and 7 to 8 h (rapid) utilizing 100 recent clinical isolates. The zone diameters for 664 disks were monitored by using the standard classification: resistant, intermediate, or susceptible. The susceptibility determination was unchanged in 558 out of 664 instances (84.0%). An analysis of the remaining 106 sets revealed that an initial interpretation of intermediate in zone size, subsequently determined resistant or susceptible, accounted for 49 of the observed differences. The reverse changes, initial resistant or susceptible subsequently classified as intermediate, accounted for 20 of the changes. In five instances the interpretation changed from susceptible to resistant; in two cases the interpretation changed from resistant to susceptible. The remaining 30 determinations were classified as indeterminant due to (i) insufficient growth at the early (7 to 8 h) determination, and to (ii) zones which were so large that they could not be measured accurately. The data indicate that zone sizes when measured to the nearest 0.1 mm can be interpreted with reasonable accuracy and the results can be available 10 to 14 h sooner.
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Selected References
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- Barry A. L., Joyce L. J., Adams A. P., Benner E. J. Rapid determination of antimicrobial susceptibility for urgent clinical situations. Am J Clin Pathol. 1973 May;59(5):693–699. doi: 10.1093/ajcp/59.5.693. [DOI] [PubMed] [Google Scholar]
- Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
- Boyle V. J., Fancher M. E., Ross R. W., Jr Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob Agents Chemother. 1973 Mar;3(3):418–424. doi: 10.1128/aac.3.3.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COOPER K. E., GILLESPIE W. A. The influence of temperature on streptomycin inhibition zones in agar cultures. J Gen Microbiol. 1952 Aug;7(1-2):1–7. doi: 10.1099/00221287-7-1-2-1. [DOI] [PubMed] [Google Scholar]
- COOPER K. E., LINTON A. H. The importance of the temperature during the early hours of incubation of agar plates in assays. J Gen Microbiol. 1952 Aug;7(1-2):8–17. doi: 10.1099/00221287-7-1-2-8. [DOI] [PubMed] [Google Scholar]
- HUMPHREY J. H., LIGHTBOWN J. W. A general theory for plate assay of antibiotics with some practical applications. J Gen Microbiol. 1952 Aug;7(1-2):129–143. doi: 10.1099/00221287-7-1-2-129. [DOI] [PubMed] [Google Scholar]
- Liberman D. F., Fitzgerald J., Robertson R. G. Rapid disk test for determining clindamycin serum levels. Antimicrob Agents Chemother. 1974 May;5(5):458–461. doi: 10.1128/aac.5.5.458. [DOI] [PMC free article] [PubMed] [Google Scholar]