Abstract
To assess their potential value as early indicators of gentamicin-induced kidney damage, lysosomal hydrolases were measured in the 24-h urines of rats receiving 30 or 60 mg of gentamicin per kg per day for 15 days. Proteinuria, urine osmolality, blood urea nitrogen, and creatinine clearance were also measured. Kidney tissue was examined by both light and electron microscopy. Beta-galactosidase, beta-n-acetyl-hexosaminidase, and alpha-fucosidase were sensitive indicators and were significantly elevated above control values by day 3 at both doses (P < 0.01). Proteinuria, urine osmolality, and tests reflecting glomerular filtration rate were later indicators of nephron damage. Changes by light microscopy were detected on day 5. Necrosis was most prominent in the proximal convoluted tubules on day 10. Electron microscopy revealed numerous cytosomes with myeloid bodies within the proximal tubular epithelium on day 5. Lysosomal enzymuria appears to be an early manifestation of gentamicin nephrotoxicity and may possibly be related to the lysosomal abnormalities seen on electron microscopy.
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- Dossetor J. B. Creatininemia versus uremia. The relative significance of blood urea nitrogen and serum creatinine concentrations in azotemia. Ann Intern Med. 1966 Dec;65(6):1287–1299. doi: 10.7326/0003-4819-65-6-1287. [DOI] [PubMed] [Google Scholar]
- Kahn T., Stein R. M. Gentamicin and renal failure. Lancet. 1972 Feb 26;1(7748):498–498. doi: 10.1016/s0140-6736(72)90165-1. [DOI] [PubMed] [Google Scholar]
- Kosek J. C., Mazze R. I., Cousins M. J. Nephrotoxicity of gentamicin. Lab Invest. 1974 Jan;30(1):48–57. [PubMed] [Google Scholar]
- MARSH W. H., FINGERHUT B., MILLER H. AUTOMATED AND MANUAL DIRECT METHODS FOR THE DETERMINATION OF BLOOD UREA. Clin Chem. 1965 Jun;11:624–627. [PubMed] [Google Scholar]
- MARTINEZ E., DOOLAN P. D. Determination of creatinine in small quantities of plasma. Observations on two methods. Clin Chem. 1960 Jun;6:233–242. [PubMed] [Google Scholar]
- Patel V., Tappel A. L., O'Brien J. S. Hyaluronidase and sulfatase deficiency in Hurler's syndrome. Biochem Med. 1970 Jun;3(6):447–457. doi: 10.1016/0006-2944(70)90037-2. [DOI] [PubMed] [Google Scholar]
- Robinson D., Price R. G., Dance N. Rat-urine glycosidases and kidney damage. Biochem J. 1967 Feb;102(2):533–538. doi: 10.1042/bj1020533. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson D., Willcox P. 4-Methylumbelliferyl phosphate as a substrate for lysosomal acid phosphatase. Biochim Biophys Acta. 1969 Sep 30;191(1):183–186. doi: 10.1016/0005-2744(69)90334-9. [DOI] [PubMed] [Google Scholar]