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. 1975 Apr;7(4):400–412. doi: 10.1128/aac.7.4.400

Antibiotic Susceptibility Testing of Gram-Negative Nonfermentative Bacilli

K A Ruddell 1, C R Anselmo 1
PMCID: PMC429152  PMID: 1147576

Abstract

A study was undertaken to determine if current methods of antibiotic susceptibility testing could be successfully applied to the gram-negative nonfermentative bacilli. Using clinical isolates and reference strains, experiments were conducted on the inherent reliability of the Bauer-Kirby method, as well as the effect of certain modifications on the method such as elimination of the 2- to 5-h incubation in broth and use of different agar media. Results obtained using these modifications were compared to the results obtained by the standard method. It was shown that the two modifications investigated had a significant effect on the interpretation of zone diameters. It was further shown that the standard Bauer-Kirby method with some exceptions correlates with minimal inhibitory concentrations as determined by broth dilution methods. Results suggest that the Bauer-Kirby method may be a reliable technique for testing the antibiotic susceptibility of the nonfermentative bacilli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler J. L., Finland M. Susceptibility of recent isolates of Pseudomonas aeruginosa to gentamicin, polymyxin, and five penicillins, with observations on the pyocin and immunotypes of the strains. Appl Microbiol. 1971 Nov;22(5):870–875. doi: 10.1128/am.22.5.870-875.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alami S. Y., Riley H. D., Jr Infections caused by mimeae, with special reference to Mima polymorpha: a review. Am J Med Sci. 1966 Nov;252(5):537–544. [PubMed] [Google Scholar]
  3. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  4. Blazevic D. J., Koepcke M. H., Matsen J. M. Quality control testing with disk antibiotic susceptibility test of Bauer-Kirby-Sherris-Turck. Am J Clin Pathol. 1972 May;57(5):592–597. doi: 10.1093/ajcp/57.5.592. [DOI] [PubMed] [Google Scholar]
  5. DALY A. K., POSTIC B., KASS E. H. Infections due to organisms of the genus Herellea. B5W and B. anitratum. Arch Intern Med. 1962 Nov;110:580–591. doi: 10.1001/archinte.1962.03620230026006. [DOI] [PubMed] [Google Scholar]
  6. Esser V. M., Elefson D. E. Experiences with the Kirby-Bauer method of antibiotic susceptibility testing. Am J Clin Pathol. 1970 Aug;54(2):193–198. doi: 10.1093/ajcp/54.2.193. [DOI] [PubMed] [Google Scholar]
  7. Gilardi G. L. Antimicrobial susceptibility as a diagnostic aid in the identification of nonfermenting gram-negative bacteria. Appl Microbiol. 1971 Nov;22(5):821–823. doi: 10.1128/am.22.5.821-823.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moody M. R., Young V. M., Kenton D. M. In vitro antibiotic susceptibility of pseudomonads other than Pseudomonas aeruginosa recovered from cancer patients. Antimicrob Agents Chemother. 1972 Nov;2(5):344–349. doi: 10.1128/aac.2.5.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Olsen H. An in vitro study of the antibiotic sensitivity of Flavobacterium meningosepticum. Acta Pathol Microbiol Scand. 1967;70(4):601–612. doi: 10.1111/j.1699-0463.1967.tb01329.x. [DOI] [PubMed] [Google Scholar]
  10. POSTIC B., FINLAND M. In vitro susceptibility of recently isolated strains of Pseudomonas aeruginosa to ten antibiotics. Am J Med Sci. 1961 Nov;242:551–559. doi: 10.1097/00000441-196111000-00004. [DOI] [PubMed] [Google Scholar]
  11. Pedersen M. M., Marso E., Pickett M. J. Nonfermentative bacilli associated with man. 3. Pathogenicity and antibiotic susceptibility. Am J Clin Pathol. 1970 Aug;54(2):178–192. doi: 10.1093/ajcp/54.2.178. [DOI] [PubMed] [Google Scholar]
  12. Petersdorf R. G., Sherris J. C. Methods and significance of in vitro testing of bacterial sensitivity to drugs. Am J Med. 1965 Nov;39(5):766–779. doi: 10.1016/0002-9343(65)90096-3. [DOI] [PubMed] [Google Scholar]
  13. Pickett M. J., Pedersen M. M. Nonfermentative bacilli associated with man. II. Detection and identification. Am J Clin Pathol. 1970 Aug;54(2):164–177. doi: 10.1093/ajcp/54.2.164. [DOI] [PubMed] [Google Scholar]
  14. Rees T. A. Antibiotic resistance in Pseudomonas aeruginosa from infections of the ear, nose and throat. Pract Otorhinolaryngol (Basel) 1971;33(6):388–393. doi: 10.1159/000275020. [DOI] [PubMed] [Google Scholar]
  15. Russell J. P. Antibiotic sensitivity testing. Postgrad Med. 1969 Jan;45(1):51–54. doi: 10.1080/00325481.1969.11696981. [DOI] [PubMed] [Google Scholar]
  16. SPAULDING E. H., ANDERSON T. G. Selection of antimicrobial agents by laboratory means. J Am Med Assoc. 1951 Dec 1;147(14):1336–1341. doi: 10.1001/jama.1951.03670310026010. [DOI] [PubMed] [Google Scholar]
  17. Smith C. B., Finland M. Carbenicillin: activity in vitro and absorption and excretion in normal young men. Appl Microbiol. 1968 Nov;16(11):1753–1760. doi: 10.1128/am.16.11.1753-1760.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Von Graevenitz A., Redys J. J. Disc sensitivity as an aid in the identification of some gram-negative non-fermentative rods. Health Lab Sci. 1968 Apr;5(2):107–112. [PubMed] [Google Scholar]

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