Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1975 May;7(5):636–639. doi: 10.1128/aac.7.5.636

Inhibition of Dihydrostreptomycin Action on Mycobacterium smegmatis by Monovalent and Divalent Cation Salts

William H Beggs 1, Fred A Andrews 1
PMCID: PMC429194  PMID: 50048

Abstract

We have examined and compared the effects of monovalent and divalent cation salts on dihydrostreptomycin (DSM) action against Mycobacterium smegmatis. The Sauton synthetic liquid medium used was supplemented with test salts on the basis of ionic strength (μ). Turbidimetric growth experiments showed that 0.02 M MgSO4 (μ = 0.08) prevented growth inhibition by 0.1 μg of dihydrostreptomycin per ml, but 0.02 M NaCl (μ = 0.02) did not. However, at molarities equivalent to μ = 0.08, four monovalent cation salts, including NaCl, Na2SO4, NH4Cl, and (NH4)2SO4, all prevented inhibition by dihydrostreptomycin. When magnesium and sodium salts were compared at μ = 0.02, 0.04, and 0.05, two distinct growth protective patterns were seen. These data were indicative of two different mechanisms of dihydrostreptomycin antagnosim by salts; the first being divalent cation and concentration dependent, and the second being nonspecific and ionic strength dependent. Viability studies supported the existence of two mechanisms.

Full text

PDF
636

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beggs W. H., Andrews F. A. Chemical characterization of ethambutol binding to Mycobacterium smegmatis. Antimicrob Agents Chemother. 1974 Mar;5(3):234–239. doi: 10.1128/aac.5.3.234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beggs W. H., Andrews F. A. Nonspecificity in the divalent cation antagonism of dihydrostreptomycin action on Mycobacterium smegmatis. Res Commun Chem Pathol Pharmacol. 1975 Jan;10(1):185–188. [PubMed] [Google Scholar]
  3. Beggs W. H., Williams N. E. Protection of Mycobacterium smegmatis from Ethambutol and Streptomycin Inhibition by MgSO(4) and Polyamines. Infect Immun. 1971 Mar;3(3):496–497. doi: 10.1128/iai.3.3.496-497.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beggs W. H., Williams N. E. Streptomycin uptake by Mycobacterium tuberculosis. Appl Microbiol. 1971 Apr;21(4):751–753. doi: 10.1128/am.21.4.751-753.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Berkman S., Henry R. J., Housewright R. D. Studies on Streptomycin: I. Factors Influencing the Activity of Streptomycin. J Bacteriol. 1947 May;53(5):567–574. [PMC free article] [PubMed] [Google Scholar]
  6. Chang F. N., Flaks J. G. Binding of dihydrostreptomycin to Escherichia coli ribosomes: characteristics and equilibrium of the reaction. Antimicrob Agents Chemother. 1972 Oct;2(4):294–307. doi: 10.1128/aac.2.4.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DUBIN D. T., HANCOCK R., DAVIS B. D. THE SEQUENCE OF SOME EFFECTS OF STREPTOMYCIN IN ESCHERICHIA COLI. Biochim Biophys Acta. 1963 Aug 13;74:476–489. doi: 10.1016/0006-3002(63)91390-8. [DOI] [PubMed] [Google Scholar]
  8. Davis S. D., Iannetta A. Antagonistic effect of calcium in serum on the activity of tobramycin against Pseudomonas. Antimicrob Agents Chemother. 1972 Jun;1(6):466–469. doi: 10.1128/aac.1.6.466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Donovick R., Bayan A. P., Canales P., Pansy F. The Influence of Certain Substances on the Activity of Streptomycin: III. Differential Effects of Various Electrolytes on the Action of Streptomycin. J Bacteriol. 1948 Jul;56(1):125–137. [PMC free article] [PubMed] [Google Scholar]
  10. Gilbert D. N., Kutscher E., Ireland P., Barnett J. A., Sanford J. P. Effect of the concentrations of magnesium and calcium on the in-vitro susceptibility of Pseudomonas aeruginosa to gentamicin. J Infect Dis. 1971 Dec;124 (Suppl):S37–S45. doi: 10.1093/infdis/124.supplement_1.s37. [DOI] [PubMed] [Google Scholar]
  11. HURWITZ C., ROSANO C. L. Accumulation of label from C14-streptomycin by Escherichia coli. J Bacteriol. 1962 Jun;83:1193–1201. doi: 10.1128/jb.83.6.1193-1201.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MAGER J., BENEDICT M., ARTMAN M. A common site of action of polyamines and streptomycin. Biochim Biophys Acta. 1962 Jul 30;62:202–204. doi: 10.1016/0006-3002(62)90519-x. [DOI] [PubMed] [Google Scholar]
  13. Medeiros A. A., O'Brien T. F., Wacker W. E., Yulug N. F. Effect of salt concentration on the apparent in-vitro susceptibility of Pseudomonas and other gram-negative bacilli to gentamicin. J Infect Dis. 1971 Dec;124 (Suppl):S59–S64. doi: 10.1093/infdis/124.supplement_1.s59. [DOI] [PubMed] [Google Scholar]
  14. PLOTZ P. H., DUBIN D. T., DAVIS B. D. Influence of salts on the uptake of streptomycin by Escherichia coli. Nature. 1961 Sep 23;191:1324–1325. doi: 10.1038/1911324a0. [DOI] [PubMed] [Google Scholar]
  15. RUBENIS M., KOZIJ V. M., JACKSON G. G. LABORATORY STUDIES ON GENTAMICIN. Antimicrob Agents Chemother (Bethesda) 1963;161:153–156. [PubMed] [Google Scholar]
  16. SAKURAI K., KOLB L. B., NAIMAN J. G., MARTIN J. D., Jr EFFECTS OF CALCIUM ION ON KANAMYCIN ACTIVITY. Am Surg. 1965 Mar;31:165–170. [PubMed] [Google Scholar]
  17. Zimelis V. M., Jackson G. G. Activity of aminoglycoside antibiotics aganst Pseudomonas aeruginosa: specificity and site of calcium and magnesium antagonism. J Infect Dis. 1973 Jun;127(6):663–669. doi: 10.1093/infdis/127.6.663. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES