Abstract
The effects of streptomycin on the synthesis of ribonucleic acid (RNA) and guanosine-3′-diphosphate-5′-diphosphate have been examined in the isogenic strains CP78 (rel+) and CP79 (rel−). In the absence of the required amino acids arginine, leucine, or threonine, streptomycin stimulates RNA synthesis in CP78 and this stimulation coincides with cell death. However, in the absence of histidine, also a required amino acid, streptomycin kills the cells without stimulation of RNA synthesis above that which occurs in the absence of streptomycin. In all of these instances, guanosine-3′-diphosphate-5′-diphosphate levels vary inversely with RNA synthesis, decreasing when a stimulation of RNA synthesis occurs. Streptomycin has little effect on guanosine-3′-diphosphate-5′-diphosphate levels in CP78 in the absence of histidine. Another histidine auxotroph, 15 T−H−U− (rel+), does show streptomycin-stimulated synthesis of RNA which is coincident with cell death. CP79 (rel−) is 10 times more susceptible to streptomycin than CP78 and streptomycin causes an inhibition of the relaxed synthesis of RNA. The greater susceptibility of CP79 may be due to a greater initial uptake of streptomycin by CP79.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Block R., Haseltine W. A. Thermolability of the stringent factor in rel mutants of Escherichia coli. J Mol Biol. 1973 Jul 15;77(4):625–629. doi: 10.1016/0022-2836(73)90228-3. [DOI] [PubMed] [Google Scholar]
- Cohen S. S., Hoffner N., Jansen M., Moore M., Raina A. POLYAMINES, RNA SYNTHESIS, AND STREPTOMYCIN LETHALITY IN A RELAXED MUTANT OF E. coli STRAIN 15 TAU. Proc Natl Acad Sci U S A. 1967 Mar;57(3):721–728. doi: 10.1073/pnas.57.3.721. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EIDLIC L., NEIDHARDT F. C. ROLE OF VALYL-SRNA SYNTHETASE IN ENZYME REPRESSION. Proc Natl Acad Sci U S A. 1965 Mar;53:539–543. doi: 10.1073/pnas.53.3.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FANGMAN W. L., NEIDHARDT F. C. PROTEIN AND RIBONUCLEIC ACID SYNTHESIS IN A MUTANT OF ESCHERICHIA COLI WITH AN ALTERED AMINOACYL RIBONUCLEIC ACID SYNTHETASE. J Biol Chem. 1964 Jun;239:1844–1847. [PubMed] [Google Scholar]
- Freda C. E., Cohen S. S. Nature of ribonucleic acid stimulated by streptomycin in the absence of protein synthesis. J Bacteriol. 1966 Dec;92(6):1680–1688. doi: 10.1128/jb.92.6.1680-1688.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freda C. E., Cohen S. S. Streptomycin and infection of Escherichia coli by T6r+ bacteriophage. J Bacteriol. 1966 Dec;92(6):1670–1679. doi: 10.1128/jb.92.6.1670-1679.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fukuma I., Cohen S. S. Polyamine synthesis and accumulation in Escherichia coli infected with bacteriophage R17. J Virol. 1973 Dec;12(6):1259–1264. doi: 10.1128/jvi.12.6.1259-1264.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haseltine W. A., Block R., Gilbert W., Weber K. MSI and MSII made on ribosome in idling step of protein synthesis. Nature. 1972 Aug 18;238(5364):381–384. doi: 10.1038/238381a0. [DOI] [PubMed] [Google Scholar]
- Lelong J. C., Gros D., Cousin M. A., Grunberg-Manago M., Gros F. Streptomycin induced release of fMet-tRNA from the ribosomal initiation complex. Biochem Biophys Res Commun. 1971 Feb 5;42(3):530–537. doi: 10.1016/0006-291x(71)90403-7. [DOI] [PubMed] [Google Scholar]
- Modolell J., Davis B. D. Breakdown by streptomycin of initiation complexes formed on ribosomes of Escherichia coli. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1148–1155. doi: 10.1073/pnas.67.3.1148. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pedersen F. S., Lund E., Kjeldgaard N. O. Codon specific, tRNA dependent in vitro synthesis of ppGpp and pppGpp. Nat New Biol. 1973 May 2;243(122):13–15. [PubMed] [Google Scholar]
- STERN J. L., COHEN S. S. LETHALITY AND THE STIMULATION OF RNA SYNTHESIS BY STREPTOMYCIN. Proc Natl Acad Sci U S A. 1964 May;51:859–865. doi: 10.1073/pnas.51.5.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stern J. L., Barner H. D., Cohen S. S. The lethality of streptomycin and the stimulation of RNA synthesis in the absence of protein synthesis. J Mol Biol. 1966 May;17(1):188–217. doi: 10.1016/s0022-2836(66)80103-1. [DOI] [PubMed] [Google Scholar]