Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1975 Jul;8(1):18–21. doi: 10.1128/aac.8.1.18

Effects of 5-Bromodeoxyuridine and 5-Iododeoxyuridine on a Latent Herpes Simplex Virus Infection

J Varani 1, J J Kelleher 1
PMCID: PMC429253  PMID: 1172408

Abstract

A latent herpes simplex virus infection was established in rabbit kidney cells. Treatment of the cells with 5-bromo-2-deoxyuridine after the latent infection was established had no effect on the rate of virus recovery but did extend the latent period before active virus growth resumed. In contrast to this, treatment of cells with 5-bromo-2-deoxyuridine or 5-iodo-2-deoxyuridine prior to infection with virus increased the subsequent rate of virus recovery.

Full text

PDF
18

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A. Chemical induction of focus-forming virus from nonproducer cells transformed by murine sarcoma virus. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3069–3072. doi: 10.1073/pnas.68.12.3069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aaronson S. A., Todaro G. J., Scolnick E. M. Induction of murine C-type viruses from clonal lines of virus-free BALB-3T3 cells. Science. 1971 Oct 8;174(4005):157–159. doi: 10.1126/science.174.4005.157. [DOI] [PubMed] [Google Scholar]
  3. Fogel M. Induction of virus synthesis in polyoma-transformed cells by DNA antimetabolites and by irradiation after pretreatment with 5-bromodeoxyuridine. Virology. 1972 Jul;49(1):12–22. doi: 10.1016/s0042-6822(72)80003-5. [DOI] [PubMed] [Google Scholar]
  4. Fogel M., Sachs L. The activation of virus synthesis in polyoma-transformed cells. Virology. 1969 Mar;37(3):327–334. doi: 10.1016/0042-6822(69)90216-5. [DOI] [PubMed] [Google Scholar]
  5. Gerber P. Activation of Epstein-Barr virus by 5-bromodeoxyuridine in "virus-free" human cells (complement-fixing antigen-immunofluorescence-leukocytes). Proc Natl Acad Sci U S A. 1972 Jan;69(1):83–85. doi: 10.1073/pnas.69.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glaser R., Rapp F. Rescue of Epstein-Barr virus from somatic cell hybrids of Burkitt lymphoblastoid cells. J Virol. 1972 Aug;10(2):288–296. doi: 10.1128/jvi.10.2.288-296.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HERRMAN E. C., Jr Plaque inhibition test for detection of specific inhibitors of DNA containing viruses. Proc Soc Exp Biol Med. 1961 May;107:142–145. [PubMed] [Google Scholar]
  8. Hampar B., Derge J. G., Martos L. M., Walker J. L. Synthesis of Epstein-Barr virus after activation of the viral genome in a "virus-negative" human lymphoblastoid cell (Raji) made resistant to 5-bromodeoxyuridine (thymidine kinase-virus antigen-immunofluorescence-herpesvirus fingerprints). Proc Natl Acad Sci U S A. 1972 Jan;69(1):78–82. doi: 10.1073/pnas.69.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KAUFMAN H. E. Clinical cure of herpes simplex keratitis by 5-iodo-2-deoxyuridine. Proc Soc Exp Biol Med. 1962 Feb;109:251–252. doi: 10.3181/00379727-109-27169. [DOI] [PubMed] [Google Scholar]
  10. Kelleher J. J., Varani J., Nelson W. W. Establishment of a nonproductive herpes simplex virus infection in rabbit kidney cells. Infect Immun. 1975 Jul;12(1):128–133. doi: 10.1128/iai.12.1.128-133.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klement V., Nicolson M. O., Huebner R. J. Rescue of the genome of focus forming virus from rat non-productive lines by 5'-bromodeoxyruidine. Nat New Biol. 1971 Nov 3;234(44):12–14. doi: 10.1038/newbio234012a0. [DOI] [PubMed] [Google Scholar]
  12. Lowy D. R., Rowe W. P., Teich N., Hartley J. W. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science. 1971 Oct 8;174(4005):155–156. doi: 10.1126/science.174.4005.155. [DOI] [PubMed] [Google Scholar]
  13. PERKINS E. S., WOOD R. M., SEARS M. L., PRUSOFF W. H., WELCH A. D. Anti-viral activities of several iodinated pyrimidine deoxyribonucleosides. Nature. 1962 Jun 9;194:985–986. doi: 10.1038/194985a0. [DOI] [PubMed] [Google Scholar]
  14. PRUSOFF W. H. Synthesis and biological activities of iododeoxyuridine, an analog of thymidine. Biochim Biophys Acta. 1959 Mar;32(1):295–296. doi: 10.1016/0006-3002(59)90597-9. [DOI] [PubMed] [Google Scholar]
  15. Rowe W. P. The kinetics of rescue of the murine sarcoma virus genome from a nonproducer line of transformed mouse cells. Virology. 1971 Nov;46(2):369–374. doi: 10.1016/0042-6822(71)90038-9. [DOI] [PubMed] [Google Scholar]
  16. St Jeor S., Rapp F. Cytomegalovirus replication in cells pretreated with 5-iodo-2'-deoxyuridine. J Virol. 1973 Jun;11(6):986–990. doi: 10.1128/jvi.11.6.986-990.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. St Jeor S., Rapp F. Cytomegalovirus: conversion of nonpermissive cells to a permissive state for virus replication. Science. 1973 Sep 14;181(4104):1060–1061. doi: 10.1126/science.181.4104.1060. [DOI] [PubMed] [Google Scholar]
  18. Stewart S. E., Kasnic G., Jr, Draycott C., Ben T. Activation of viruses in human tumors by 5-iododeoxyuridine and dimethyl sulfoxide. Science. 1972 Jan 14;175(4018):198–199. doi: 10.1126/science.175.4018.198. [DOI] [PubMed] [Google Scholar]
  19. Stewart S. E., Kasnic G., Jr, Draycott C., Feller W., Golden A., Mitchell E. Activation in vitro, by 5-iododeoxyuridine, of a latent virus resembling C-type virus in a human sarcoma cell line. J Natl Cancer Inst. 1972 Jan;48(1):273–277. [PubMed] [Google Scholar]
  20. Sugawara K., Mizuno F., Osato T. Epstein-Barr virus-associated antigens in non-producing clones of human lymphoblastoid cell lines. Nat New Biol. 1972 Oct 25;239(95):242–243. doi: 10.1038/newbio239242a0. [DOI] [PubMed] [Google Scholar]
  21. Sugawara K., Mizuno F., Osato T. Induction of Epstein-Barr virus-related membrane antigens by 5-iododeoxyuridine in non-producer human lymphoblastoid cells. Nat New Biol. 1973 Nov 21;246(151):70–72. doi: 10.1038/newbio246070a0. [DOI] [PubMed] [Google Scholar]
  22. Sugawara K., Osato T. Two distinct antigenic components in an Epstein-Barr virus-related early product induced by halogenated pyrimidines in non-producing human lymphoblastoid cells. Nat New Biol. 1973 Jun 13;243(128):209–210. doi: 10.1038/newbio243209a0. [DOI] [PubMed] [Google Scholar]
  23. WELCH A. D., CALABRESI P., PRUSOFF W. H., CREASEY W. A., MCCOLLUM R. W. RECENT STUDIES WITH 5-IODODEOXYURIDINE (IUDR) AND RELATED COMPOUNDS. Exp Cell Res. 1963;24:SUPPL9–SUPPL9:484. doi: 10.1016/0014-4827(63)90288-x. [DOI] [PubMed] [Google Scholar]
  24. Weiss R. A., Friis R. R., Katz E., Vogt P. K. Induction of avian tumor viruses in normal cells by physical and chemical carcinogens. Virology. 1971 Dec;46(3):920–938. doi: 10.1016/0042-6822(71)90091-2. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES