Abstract
Calcium elenolate inactivates all myxoviruses so far tested. The pH of the reaction mixture is less critical for myxovirus inactivation than that required for coxsackie A-21 virus; the myxoviruses are inactivated at a broad spectrum of pH with the maximum activity occurring at a pH below 7.0. The infectivity of the virus is more susceptible to the action of calcium elenolate than is either the neuraminidase activity or the hemagglutinin. The inactivation of Newcastle disease virus by calcium elenolate also destroys the ability of the virus to induce interferon formation in cell culture and in mice.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Babiker H. A., Rott R. Plaque formation by influenza viruses in monolayers of chicken kidney cells. J Gen Virol. 1968 Sep;3(2):285–287. doi: 10.1099/0022-1317-3-2-285. [DOI] [PubMed] [Google Scholar]
- Dianzani F., Gagnoni S., Buckler C. E., Baron S. Studies of the induction of interferon system by nonreplicating Newcastle disease virus. Proc Soc Exp Biol Med. 1970 Jan;133(1):324–328. doi: 10.3181/00379727-133-34467. [DOI] [PubMed] [Google Scholar]
- Finter N. B. Quantitative hemadsorption, a new assay technique. II. Assay of neutralizing antibodies to hemadsorbing viruses. J Immunol. 1967 Jan;98(1):88–93. [PubMed] [Google Scholar]
- Gandhi S. S., Burke D. C. Interferon production by myxoviruses in chick embryo cells. J Gen Virol. 1970 Jan;6(1):95–103. doi: 10.1099/0022-1317-6-1-95. [DOI] [PubMed] [Google Scholar]
- Goedemans W. T., Peters A. Quantitative haemadsorption. A fast and reliable screening test for anti-myxoviral agents. Arch Gesamte Virusforsch. 1968;23(4):326–331. doi: 10.1007/BF01242128. [DOI] [PubMed] [Google Scholar]
- Hirschman S. Z. Inactivation of DNA polymerases of murine leukaemia viruses by calcium elenolate. Nat New Biol. 1972 Aug 30;238(87):277–279. doi: 10.1038/newbio238277a0. [DOI] [PubMed] [Google Scholar]
- Kelly R. C., Schletter I. Total synthesis of dl-methyl elenolate. J Am Chem Soc. 1973 Oct 17;95(21):7156–7157. doi: 10.1021/ja00802a043. [DOI] [PubMed] [Google Scholar]
- MacKellar F. A., Kelly R. C., van Tamelen E. E., Dorschel C. Structure and stereochemistry of elenolic acid. J Am Chem Soc. 1973 Oct 17;95(21):7155–7156. doi: 10.1021/ja00802a042. [DOI] [PubMed] [Google Scholar]
- Sheaff E. T., Meager A., Burke D. C. Factors involved in the producton of interferon by inactivated Newcastle disease virus. J Gen Virol. 1972 Nov;17(2):163–175. doi: 10.1099/0022-1317-17-2-163. [DOI] [PubMed] [Google Scholar]
- Skehel J. J., Burke D. C. Interferon production by Semliki Forest virus inactivated with hydroxylamine. J Gen Virol. 1968 Jul;3(1):35–42. doi: 10.1099/0022-1317-3-1-35. [DOI] [PubMed] [Google Scholar]
- WAGNER R. R. Biological studies of interferon. I. Suppression of cellular infection with eastern equine encephalomyelitis virus. Virology. 1961 Mar;13:323–337. doi: 10.1016/0042-6822(61)90152-0. [DOI] [PubMed] [Google Scholar]
- WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
- Youngner J. S., Scott A. W., Hallum J. V., Stinebring W. R. Interferon production by inactivated Newcastle disease virus in cell cultures and in mice. J Bacteriol. 1966 Oct;92(4):862–868. doi: 10.1128/jb.92.4.862-868.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]