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. 1975 Aug;8(2):222–223. doi: 10.1128/aac.8.2.222

Properties of the Gentamicin Acetyltransferase Enzyme and Application to the Assay of Aminoglycoside Antibiotics

J M Broughall 1, D S Reeves 1
PMCID: PMC429292  PMID: 1101816

Abstract

The acetyltransferase technique of serum gentamicin assay was demonstrated to be specific for aminoglycoside antibiotics. The enzyme involved was isolated and stabilized by lyophilization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Broughall J. M., Reeves D. S. The acetyltransferase enzyme method for the assay of serum gentamicon concentrations and a comparison with other methods. J Clin Pathol. 1975 Feb;28(2):140–145. doi: 10.1136/jcp.28.2.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Haas M. J., Davies J. Enzymatic acetylation as a means of determining serum aminoglycoside concentrations. Antimicrob Agents Chemother. 1973 Oct;4(4):497–499. doi: 10.1128/aac.4.4.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Holmes R. K., Sanford J. P. Enzymatic assay for gentamicin and related aminoglycoside antibiotics. J Infect Dis. 1974 May;129(5):519–527. doi: 10.1093/infdis/129.5.519. [DOI] [PubMed] [Google Scholar]
  4. Phillips I., Warren C., Smith S. E. Serum gentamicin assay: a comparison and assessment of different methods. J Clin Pathol. 1974 Jun;27(6):447–451. doi: 10.1136/jcp.27.6.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Reeves D. S., Holt H. A. Resolution of antibiotic mixtures in serum samples by high-voltage electrophoresis. J Clin Pathol. 1975 Jun;28(6):435–442. doi: 10.1136/jcp.28.6.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Smith A. L., Waitz J. A., Smith D. H., Oden E. M., Emerson B. B. Comparison of enzymatic and microbiological gentamicin assays. Antimicrob Agents Chemother. 1974 Sep;6(3):316–319. doi: 10.1128/aac.6.3.316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Smith D. H., Van Otto B., Smith A. L. A rapid chemical assay for gentamicin. N Engl J Med. 1972 Mar 16;286(11):583–586. doi: 10.1056/NEJM197203162861106. [DOI] [PubMed] [Google Scholar]
  8. ten Krooden E., Darrell J. H. Rapid gentamicin assay by enzymatic adenylylation. J Clin Pathol. 1974 Jun;27(6):452–456. doi: 10.1136/jcp.27.6.452. [DOI] [PMC free article] [PubMed] [Google Scholar]

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