Abstract
An improved method for experimental keratitis due to Pseudomonas aeruginosa is described. Essential features of the method are use of inbred guinea pigs, intracorneal injection of bacteria, subconjunctival injection of antibiotics, “blind” evaluation of results, and statistical analysis of data. Untreated ocular infections were most severe 5 to 7 days after infection. Sterilized bacterial suspensions caused no abnormalities on day 5. Tobramycin and polymyxin B were more active than gentamicin against two strains of Pseudomonas. This model is suitable for many types of quantitative studies on experimental keratitis.
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- Bohigian G., Okumoto M., Valenton M. Experimental pseudomonas keratitis. Treatment with and evaluation of carbenicillin and gentamicin in combination. Arch Ophthalmol. 1971 Oct;86(4):432–437. doi: 10.1001/archopht.1971.01000010434013. [DOI] [PubMed] [Google Scholar]
- Davis S. D. Activity of gentamicin, tobramycin, polymyxin B, and colistimethate in mouse protection tests with Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1975 Jul;8(1):50–53. doi: 10.1128/aac.8.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davis S. D. Dissociation between results of in vitro and in vivo antibiotic susceptibility tests for some strains of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1974 Mar;5(3):281–288. doi: 10.1128/aac.5.3.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fisher M. W., Devlin H. B., Gnabasik F. J. New immunotype schema for Pseudomonas aeruginosa based on protective antigens. J Bacteriol. 1969 May;98(2):835–836. doi: 10.1128/jb.98.2.835-836.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hessburg P. C. Management of pseudomonas keratitis. Surv Ophthalmol. 1969 Jul;14(1):43–54. [PubMed] [Google Scholar]
- Hessburg P. C., Truant J. P., Penn W. P. Treatment of Pseudomonas keratitis in rabbits. Am J Ophthalmol. 1966 Jan;61(1):49–54. doi: 10.1016/0002-9394(66)90746-x. [DOI] [PubMed] [Google Scholar]
- Laibson P. R. Cornea and sclera. Arch Ophthalmol. 1972 Nov;88(5):553–574. doi: 10.1001/archopht.1972.01000030555018. [DOI] [PubMed] [Google Scholar]
- Reller L. B., Schoenknecht F. D., Kenny M. A., Sherris J. C. Antibiotic susceptibility testing of Pseudomonas aeruginosa: selection of a control strain and criteria for magnesium and calcium content in media. J Infect Dis. 1974 Nov;130(5):454–463. doi: 10.1093/infdis/130.5.454. [DOI] [PubMed] [Google Scholar]
- Smith D. H., Van Otto B., Smith A. L. A rapid chemical assay for gentamicin. N Engl J Med. 1972 Mar 16;286(11):583–586. doi: 10.1056/NEJM197203162861106. [DOI] [PubMed] [Google Scholar]