Hydrogen peroxide induces vasorelaxation by enhancing 4-aminopyridine-sensitive Kv currents through S-glutathionylation

Abstract

Hydrogen peroxide (H₂O₂) is an endothelium-derived hyperpolarizing factor. Since opposing vasoactive effects have been reported for H₂O₂ depending on the vascular bed and experimental conditions, this study was performed to assess whether H₂O₂ acts as a vasodilator in the rat mesenteric artery and, if so, to determine the underlying mechanisms. H₂O₂ elicited concentration-dependent relaxation in mesenteric arteries precontracted with norepinephrine. The vasodilatory effect of H₂O₂ was reversed by treatment with dithiothreitol. H₂O₂-elicited vasodilation was significantly reduced by blocking 4-aminopyridine (4-AP)-sensitive Kv channels, but it was resistant to blockers of big-conductance Ca²⁺-activated K⁺ channels and inward rectifier K⁺ channels. A patch-clamp study in mesenteric arterial smooth muscle cells (MASMCs) showed that H₂O₂ increased Kv currents in a concentration-dependent manner. H₂O₂ speeded up Kv channel activation and shifted steady state activation to hyperpolarizing potentials. Similar channel activation was seen with oxidized glutathione (GSSG). The H₂O₂-mediated channel activation was prevented by glutathione reductase. Consistent with S-glutathionylation, streptavidin pull-down assays with biotinylated glutathione ethyl ester showed incorporation of glutathione (GSH) in the Kv channel proteins in the presence of H₂O₂. Interestingly, conditions of increased oxidative stress within MASMCs impaired the capacity of H₂O₂ to stimulate Kv channels. Not only was the H₂O₂ stimulatory effect much weaker, but the inhibitory effect of H₂O₂ was unmasked. These data suggest that H₂O₂ activates 4-AP-sensitive Kv channels, possibly through S-glutathionylation, which elicits smooth muscle relaxation in rat mesenteric arteries. Furthermore, our results support the idea that the basal redox status of MASMCs determines the response of Kv currents to H₂O₂.

Electronic supplementary material

The online version of this article (doi:10.1007/s00424-014-1513-3) contains supplementary material, which is available to authorized users.

Introduction

Reactive oxygen species (ROS) are detrimental to biological processes and contribute to disease conditions such as inflammation, ischemia–reperfusion injury, atherosclerosis, diabetes mellitus, and hypertension. However, some ROS like hydrogen peroxide (H₂O₂) may act as physiological signaling molecules and contribute to biologically beneficial processes [34, 72].

H₂O₂ has been suggested to be an endothelium-derived hyperpolarizing factor (EDHF) [69], a major component of endothelium-dependent relaxation in resistance-sized arteries [70]. The cellular and molecular mechanisms by which H₂O₂ elicits vasodilation remain to be determined, although smooth muscle hyperpolarization seems to be required [58]. The vascular smooth muscle cells functionally express four different K⁺ channels [4, 33, 43, 45, 49], namely, voltage-gated (Kv), Ca²⁺-activated (K_Ca), ATP-sensitive (K_ATP), and inward rectifier K⁺ (Kir) channels. The K⁺ channels are thought to play an important role in maintaining the membrane potential of vascular myocytes [45] and have been implicated in H₂O₂-induced smooth muscle relaxation. However, there is no general agreement on the effects of H₂O₂ on K⁺ channels in smooth muscle. Several studies have identified K_Ca channels as putative targets that are activated in the process of H₂O₂-induced vasodilation [81], while some other groups indicate that H₂O₂ induced a vasorelaxation through opening of ATP-dependent K⁺ (K_ATP) channels [74]. The Kv channel is also suggested to be a target of H₂O₂, which activates Kv channels in coronary and pulmonary vascular smooth muscles [44, 54, 57]. However, Kv channels are inhibited by H₂O₂ in ductus arteriosus smooth muscles [2]. Studies using cloned Kv1.5, a major component of Kv current in coronary arteries, show that H₂O₂ increases Kv1.5 current for voltages < +20 mV but decreases it for high depolarizing voltages [12]. It is still uncertain whether H₂O₂ acts as a vasodilator. Studies have reported H₂O₂ as a vasoconstrictor [30, 67] and vasodilator [29, 73], or both [18, 38]. These differences may depend on experimental design and the specific vascular bed or vessel being studied [42, 47].

H₂O₂ is a small stable molecule carrying no charge, which allows it to readily cross membranes and travel freely to targets within cells [25]. Various cellular modifications occur with the increase in H₂O₂, and increasing evidence suggests that S-glutathionylation predominates in myocytes because glutathione (GSH) is the most abundant, low molecular mass, reducing equivalent [59]. H₂O₂ is capable of oxidizing the thiol groups of cysteine residues to form disulfide bonds with GSH (S-glutathionylation). S-glutathionylated proteins result from thiol/disulfide exchange between protein thiols and oxidized form of glutathione (GSSG) or S-glutathionylated protein. Modulation of protein activity by S-glutathionylation is a newly recognized posttranslational regulatory mechanism [20, 21]. This process results in major changes to protein conformation and function [22]. The Kir6.1/SUR2B channel and Cav1.2 channel are subject to S-glutathionylation induced by H₂O₂ [63, 71, 78].

In a previous study, we demonstrated that in mesenteric arteries, the 4-aminopyridine (4-AP)-sensitive Kv currents play a critical role in the regulation of smooth muscle resting membrane potential (Em) and vascular tone [3, 66]. In this study, we examined the hypothesis that H₂O₂ relaxes rat mesenteric arteries by S-glutathionylation-dependent activation of 4-AP-sensitive Kv channels. We performed studies using an approach of combined molecular biology, electrophysiology, and isometric organ chamber mechanics. Our results show that H₂O₂ enhanced the activity of 4-AP-sensitive Kv channels, possibly through S-glutathionylation, leading to vasorelaxation in the mesenteric artery. We also present evidence to show that Kv channels under conditions of persistent oxidative stress were not activated, but rather inhibited by the addition of H₂O₂, suggesting that H₂O₂ may act as a vasoconstrictor under certain pathological conditions.

Methods

Tissue and cell preparation

Male Sprague–Dawley (SD) rats (9–11 weeks old) were used for the experiments. All experiments were conducted in accordance with the National Institutes of Health guidelines for the care and use of animals, and the Institutional Animal Care and Use Committee of Konkuk University approved this study. Rats were euthanized by exposure to a rising concentration of carbon dioxide or exsanguinated by cutting the carotid arteries under deep ketamine–xylazine anesthesia. Single-cell suspensions of mesenteric arterial smooth muscle cells (MASMCs) were prepared as described previously [3]. Briefly, the second- to fourth-order branches of the superior mesenteric arteries were carefully removed and placed in normal Tyrode (NT) solution (143 mM NaCl, 5.4 mM KCl, 0.33 mM NaH₂PO₄, 1.8 mM CaCl₂, 0.5 mM MgCl₂, 5 mM hydroxyethyl piperazineethanesulfonic acid (HEPES), and 11 mM glucose, adjusted to pH 7.4 with NaOH). The arteries were cut into small pieces and transferred to a digestion solution. The tissue was first digested for 15 min in Ca²⁺-free normal NT solution containing 1 mg/mL papain (Sigma Chemical, St. Louis, MO, USA), 1 mg/mL bovine serum albumin, and 1 mg/mL dithiothreitol. The nominally Ca²⁺-free NT was prepared by omitting 1.8 mM CaCl₂ from the NT solution. Subsequently, the tissue sample was incubated for 25 min in a second digestion solution, in which 3 mg/mL collagenase (Wako, Osaka, Japan) replaced papain. Following enzyme treatment, the cells were isolated by gentle agitation with a fire-polished glass pipette in Ca²⁺-free NT solution.

Solutions and drugs

NT was used as the bathing solution for the patch-clamp experiments. The pipette internal solution contained 140 mM KCl, 5 mM NaCl, 5 mM MgATP, 10 mM HEPES, and 10 mM 1,2-bis(aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid, adjusted to pH 7.2 with KOH. Bicarbonate-buffered physiological salt solution (PSS) was used as the bath solution for the organ chamber mechanics experiments. The PSS was composed of 136.9 mM NaCl, 5.4 mM KCl, 1.5 mM CaCl₂, 1.0 mM MgCl₂, 23.8 mM NaHCO₃, and 0.01 mM EDTA. All chemicals, including H₂O₂, GSH, and GSSG, were purchased from Sigma.

Electrophysiological recordings

We used the conventional whole-cell configuration of the patch-clamp technique [3] to record membrane currents and Em. EPC8 (HEKA, Mahone Bay, Nova Scotia, Canada) patch-clamp amplifier with a DAQPad-6070E interface (National Instrument, Austin, TX, USA) or an Axopatch 200B patch-clamp amplifier with a DigiData 1200 interface (Axon Instruments, Foster City, CA, USA) was used. Data were digitized with custom-built software (R-clamp, by Dr. SY Ryu) or with pClamp6 software (Axon Instruments) at a sampling rate of 1–10 kHz. The data were low-pass filtered at 1 kHz and saved for analysis. Voltage pulse generation was also controlled by R-clamp software and pClamp6. Patch pipettes were pulled from borosilicate capillary tubes (Clark Electromedical Instruments, Pangbourne, UK) using a puller (PP-83; Narishige, Tokyo, Japan). We used patch pipettes with a resistance of 2–4 MΩ when filled with the abovementioned pipette solution. Recordings were started at least 7 min after establishing the whole-cell configuration to allow adequate cell dialysis of the pipette solution. All experiments were carried out at room temperature (20–25 °C).

Kv currents were elicited by depolarizing steps between −60 and +50 mV (200 ms duration) from a holding potential of −70 mV. Tetraethylammonium (TEA, 1 mM) was added to all bath solutions during the patch-clamp study to prevent activation of big-conductance K_Ca (BK_Ca) channels. Additionally, specific activation of the Kv current was confirmed using 4-AP at the end of the experiments. Conductance–voltage (G–V) relationships were plotted using steady state current amplitudes divided by driving force (Em–Erev, where Erev is the reversal potential of the Kv current). The normalized conductance was fit using Origin 6.0 software to the Boltzmann equation.

Organ chamber isometric contraction measurements

The mesenteric arterial rings were mounted vertically on two L-shaped stainless steel wires in a 3-mL tissue chamber for the tension measurements. One wire was attached to a micromanipulator and the other to an isometric force transducer (FT03; Grass, West Warwick, RI, USA). Changes in isometric force were digitally acquired at 1 Hz with a PowerLab data acquisition system (AD Instruments, Colorado Springs, CO, USA). Resting tension was set to 1 g using the micromanipulator. After a 60-min equilibration under resting tension in a tissue chamber filled with PSS, the rings were sequentially exposed to 70 mM KCl–PSS (10 min) and PSS (15 min) three times for stabilization. The high KCl (70 mM)–PSS was prepared by replacing NaCl with equimolar KCl in PSS. Bath solutions were thermostatically controlled at 37 °C and were continuously saturated with a mixture of 95 % O₂ in 5 % CO₂ to achieve pH 7.4.

Western blot

Primary cultures of rat MASMCs between six and ten passages were used for Western blot. MASMCs were isolated from SD rats and cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10 % fetal bovine serum (FBS) and 1 % penicillin–streptomycin. The cells were grown to 80 % confluence and starved in DMEM without FBS for 12 to 24 h prior to experiments. After starvation, cells were treated with H₂O₂ for 10 min at 37 °C. The cells were then washed twice with phosphate-buffered saline (PBS) and lysed using RIPA buffer (TNT Research, Seoul, South Korea). Samples were run on an 8 % SDS–polyacrylamide nonreducing gel and then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Bedford, MA, USA). Rabbit primary antibodies against Kv1.2, Kv1.5, and Kv 2.1 (1:500; Alomone Lab) and secondary antibodies conjugated with horseradish peroxidase were used in the Western blot (1:2,000; Cell Signaling Technology, Danvers, MA, USA). Signals were visualized using Las-4000 (Fuji Film, Tokyo, Japan).

Streptavidin pull-down assay

The culture medium was replaced with fresh medium 2 h before experiments. Biotinylated glutathione ethyl ester (BioGEE; 100 μM; Invitrogen, Carlsbad, CA, USA) was added to the medium and incubated for 1 h, followed by H₂O₂ (0.1 or 10 mM) challenges for 10 min. Biotin–GSH-conjugated proteins were pulled down using Dynabeads streptavidin according to the methods provided by Invitrogen. Dynabeads streptavidin was washed thrice with PBS before conjugation with biotin. Samples were then mixed with beads and incubated at room temperature with gentle rotation for 30 min. A magnet was used to separate the biotinylated molecule–bead complex. The supernatant containing unlabeled proteins was discarded, and the pellet was resuspended, followed by washes with PBS. The biotinylated molecule–bead complex was boiled with loading buffer for 7 min for Western blotting.

Data analysis

The Origin 6.0 software (Microcal Software, Inc., Northampton, MA, USA) was used for data analysis. Activation kinetics was calculated by fitting the data to a single exponential decay function. The time course of current inactivation was also fit to a single exponential function. The results are shown as mean ± standard error. Paired or independent Student’s t tests were used to test for significance, and p < 0.05 was regarded as significant. We performed one-way repeated measures ANOVA and Holm–Sidak test in order to examine the statistical significance of data shown in Fig. 3b and one-way ANOVA and Tukey’s test for Fig. 5c using SigmaPlot 12.5.

Fig. 3.

H₂O₂ shifts G–V curves for Kv channels to the left and speeds up channel activation. a G–V curves for K_V channels, before and after H₂O₂ (0.1–10 mM) treatment. Smooth curves were fitted using the Boltzmann function. b V _1/2 obtained from the Boltzmann function is plotted against H₂O₂ concentration. One-way repeated measures ANOVA and Holm–Sidak test (α = 0.01, p < 0.05). Statistically distinct groups are indicated by letters. c Representative traces at +40 mV show that H₂O₂ (10 mM) accelerates Kv current activation. Activation of K_V currents was fit with a single exponential function. Fits to original traces and time constant (τ) values are shown. d Time constants are presented as a function of the pulse potential in control (filled black square) and following application of 10 mM H₂O₂ (filled red circle) (*p < 0.05; **p < 0.01; ***p < 0.001 vs. control)

Fig. 5.

GSSG increases Kv currents in rat MASMCs. a Effects of adding GSSG in the pipette solution on Kv currents and on the action of H₂O₂. Averaged I–V curves before (filled black square) and after bath application of 0.1 mM H₂O₂ (filled red circle) or 10 mM H₂O₂ (filled blue triangle) in cells with GSSG in the pipette are shown. Gray line indicates the averaged I–V relationship with control pipette solution (redrawn from Fig. 2b for comparison). H₂O₂ was added cumulatively (n = 12). Note that Kv currents with pipette solution containing GSSG (filled black square) are much larger than those with control pipette solution (gray line). b Comparative data of Kv current densities measured at +40 mV for H₂O₂ effects in control pipette solution (filled gray circle; n = 21) with that in the pipette containing 10 mM GSSG (empty circle; n = 12). p < 0.01 vs. basal condition (control pipette solution); p = NS vs. basal condition (GSSG in the pipette). c Time constants recorded with GSSG pipette are presented as a function of the pulse potential before (filled black square) and after application of 10 mM H₂O₂ (filled blue triangle) (n = 8). Time constants recorded with control pipette (empty square and empty triangle) are redrawn from Fig. 3d for comparison (n = 16). One-way ANOVA and Tukey’s test, *p < 0.05; **p < 0.01; ***p < 0.001 vs. all other groups; ^# p < 0.05 vs. GSSG pipette groups; ^§ p < 0.05 vs. GSSG alone. d, e Effects of GSH reductase (0.2 units/mL) plus NADPH (d) on Kv currents and on the action of H₂O₂. NADPH alone had no effect (e). Averaged I–V curves before (filled black square) and after bath application of 0.1 mM H₂O₂ (filled red circle) and 10 mM H₂O₂ (filled blue triangle) are shown. f Comparative data between Kv current densities measured at +40 mV for H₂O₂ effects in the pipette containing NADPH alone (filled black circle; n = 11) and those in the pipette containing NADPH plus GSH reductase (empty circle; n = 11). p = NS vs. basal condition (NADPH plus GSH reductase)

Results

H₂O₂ causes relaxation of the precontracted mesenteric arterial rings by redox-dependent alterations

We used isometric organ chamber mechanics to examine whether H₂O₂ relaxes rat mesenteric arteries. We used arterial rings without intact endothelium. The arterial rings were precontracted with norepinephrine (NE) (1 μM). H₂O₂ induced a concentration-dependent relaxation in the precontracted mesenteric arterial rings (Fig. 1a, c, d). We determined whether a thiol-specific reducing agent, DL-dithiothreitol (DTT), could reverse H₂O₂-induced relaxation. Pretreatment with 1 mM DTT almost completely prevented the relaxation by 1 mM H₂O₂ (Fig. 1b, c). The addition of DTT to vascular rings, in the absence of contractile agonist, did not affect resting tension (0.23 ± 0.19 vs. 0.22 ± 0.19 g before and after adding 1 mM DTT, respectively; n = 8). These data indicate that the effect of DTT is specific for H₂O₂-induced relaxation and suggest that thiol groups in smooth muscle are targets of H₂O₂ signaling. TEA was used to assess the contribution of the K⁺ channels to H₂O₂-induced smooth muscle relaxation. At a concentration of 1 mM, TEA is reported to be relatively specific for BK_Ca channels and has little effect on voltage-dependent K⁺ channels [65]. One millimolar TEA (Fig. 1d; n = 6) did not significantly inhibit the relaxation caused by H₂O₂ (compared with control; n = 6). BaCl₂ (100 μM), a blocker of Kir, did not affect the H₂O₂-induced relaxation either (Fig. 1d). We attempted similar experiments with 10 mM 4-AP, a known Kv channel blocker; 4-AP significantly attenuated H₂O₂-induced relaxation (Fig. 1d, n = 6). These data suggest that 4-AP-sensitive Kv channels mediate the vasodilation by H₂O₂ in the mesenteric artery.

Fig. 1.

Effects of H₂O₂ on NE-precontracted mesenteric arterial rings under control and DTT-pretreated conditions. a Isometric tension recordings showing the effects of increasing concentrations of H₂O₂ (0.1–10 mM) on mesenteric arteries precontracted with NE. b Pretreatment with DTT inhibited the H₂O₂-induced relaxation. c H₂O₂-induced relaxation under control and DTT-pretreated conditions. The H₂O₂ effect was blocked by DTT (*p < 0.05 vs. control condition). d Summary of the effects of various potassium current blockers on the H₂O₂-induced relaxation (***p < 0.001 vs. control)

Effects of H₂O₂ on Kv currents in rat MASMCs

To examine whether H₂O₂ activated 4-AP-sensitive Kv currents, we recorded Kv currents using the conventional whole-cell, patch-clamp technique with depolarizing voltage steps as described previously [3] (Fig. 2). Cells were held at −70 mV to remove voltage-dependent channel inhibition, and membrane potential was stepped from −60 to +50 mV in 10-mV increments. Cumulative application of H₂O₂ superfusion (5 min at each concentration) increased Kv currents (Fig. 2a). Addition of 10 mM 4-AP to the bath reduced current magnitude below the baseline level, indicating that 4-AP-sensitive Kv channels are responsible for the outward current. H₂O₂-induced Kv current modulation was concentration-dependent; 0.1, 1, and 10 mM H₂O₂ induced an increase of Kv current amplitude at +40 mV to 11.4 ± 2.9, 47.5 ± 7.7, and 127.4 ± 23.8 %, respectively (Fig. 2b, c). Analysis of I–V relationship also indicated that the effect of H₂O₂ on current becomes significant at −20 mV. The degree of activation of the steady state Kv currents by H₂O₂ was large in the negative voltage range compared to those at potentials positive to 0 mV (Fig. 2c). This indicates that H₂O₂ can act as a potent modulator of the Kv channel function in rat MASMCs within the range of physiologically relevant voltages. H₂O₂, even at 10 mM, did not result in any nonspecific effects due to cellular damage. In all cells tested, neither access resistance nor leak current was significantly altered (Fig. 2a, b).

Fig. 2.

H₂O₂ increases Kv currents in MASMCs in a concentration-dependent manner. a Representative current traces illustrating the effect of H₂O₂ (0.1–10 mM) on whole-cell Kv currents. Deactivating tail currents were observed at −40 mV, following various test potentials, indicative of Kv channel activation. Application of 4-AP (10 mM) reduced current below the baseline level. b Current–voltage (I–V) relationship with H₂O₂ treatment (0.1–10 mM) (n = 19 MASMCs; **p < 0.01 vs. control; ***p < 0.001 vs. control). c H₂O₂-induced activation of Kv current. Amplitudes of Kv currents were measured at +40 mV. The magnitude of activation with various concentrations of H₂O₂ was plotted against Em (n = 19 for each concentration). Insets show the indicated graph with expanded scale

We then examined whether H₂O₂ influences voltage dependence of activation and activation kinetics of the putative Kv current (Fig. 3). We noticed that the conductance–voltage (G–V) curves of the Kv channel were significantly shifted to more negative potentials after H₂O₂ application (Fig. 3a), i.e., the channel was now activated at a more hyperpolarized potential. V _1/2 (midpoint of the G–V curve) in control and 0.1, 1, and 10 mM H₂O₂ were 7.6 ± 1.0, 2.7 ± 1.4, −3.1 ± 1.3, and −1.7 ± 1.8 mV, respectively (n = 14; Fig. 3b). The slope factors were unaffected (Fig. 3a). H₂O₂ also had an important effect on the time course of activation of the Kv channel (Fig. 3c). For example, for a −20-mV depolarizing pulse, the time constant of activation decreased by 60 %. Similar effects were observed for all depolarizing pulses tested (n = 16, Fig. 3d), suggesting that H₂O₂ speeds up the activation of Kv currents. The data from Figs. 2 and 3 (i.e., 4-AP sensitivity, voltage dependence, and kinetics) suggest that the currents modulated by H₂O₂ are 4-AP-sensitive K_V currents.

Biochemical evidence for the Kv channel S-glutathionylation by H₂O₂

To reveal the mechanism(s) by which H₂O₂ increases the voltage sensitivity and activation kinetics of 4-AP-sensitive Kv channels, we examined whether the Kv channel proteins from rat MASMCs can be glutathionylated after exposure to H₂O₂. It has been reported that Kv1.2 and Kv1.5 channels are molecular identities of 4-AP-sensitive Kv channels in vascular smooth muscle cells. In addition, a recent study reported that Kv2.1 and Kv 9.3 channels are oxygen-sensitive K⁺ channels in the pulmonary artery. Since a previous study confirmed the presence of Kv1.2, Kv1.5, and Kv2.1 channel proteins in isolated MASMCs by immunocytochemistry [75], we examined the S-glutathionylation of these channel proteins by using a streptavidin pull-down assay. Primarily cultured rat MASMCs were loaded with BioGEE (250 μM) for 1 h, followed by H₂O₂ (0.1–10 mM) challenge for 10 min, as described previously [82]. Strong Kv1.2 (75 kDa), Kv1.5 (67 kDa), and Kv2.1 (95 kDa) bands were detected in the whole-cell lysates (Fig. 4, lower panel). If BioGEE was incorporated into channel proteins, streptavidin beads should pull down the channel protein–BioGEE complex, which would be further detected by channel protein antibodies in a Western blot. In contrast, if the channel proteins were not glutathionylated, the binding of the channel protein to BioGEE should decrease, resulting in a weaker band or even no band in the Western blot. In the streptavidin pull-down experiments, the immunoreactivity of Kv2.1 was significantly increased in the cell lysate pretreated with H₂O₂, compared to control cells (Fig. 4, upper panel). After streptavidin pull-down, immunoreactivities of Kv1.2 and Kv1.5 were not detectable. Western blotting of whole lysates verified Kv1.2 and Kv1.5 protein expression. Similar results were obtained using immunoprecipitation with anti-GSH, followed by immunoblot with antibodies against Kv1.2, Kv1.5, and Kv2.1 (data not shown).

Fig. 4.

S-glutathionylation of the Kv 2.1 channel after exposure to H₂O₂. a Kv 2.1 channels were detected only from samples that were obtained from the cells treated with both BioGEE and H₂O₂ in the streptavidin pull-down assay. In the streptavidin pull-down assay, Kv1.2 and Kv1.5 channels were not detected even from samples that were obtained from cells treated with both BioGEE and H₂O₂. b Kv channel subunits were detected through conventional Western blot using rat mesenteric arterial smooth muscle cell (MASMC) primary cultures pretreated with various concentrations of H₂O₂ and untreated control. Band density did not change with H₂O₂ treatment. The results are representative examples of three independent experiments

S-glutathionylation mediates activation of Kv currents by H₂O₂

GSSG causes S-glutathionylation [20]. We examined whether GSSG directly increased Kv currents. We established the conventional whole-cell configuration to deliver GSSG to the cytosol, as done in a previous study [68]. Current recordings usually started 10 min after the whole-cell configuration was made. Intracellular loading of 10 mM GSSG via a patch pipette significantly increased the Kv current (Fig. 5a). At +40 mV, Kv current densities in the absence and presence of GSSG were 17.7 ± 1.5 pA/pF (n = 19) and 37.8 ± 4.3 pA/pF (n = 21, p < 0.01), respectively (Fig. 5b). Furthermore, with GSSG in the pipette, subsequent application of H₂O₂ had no effect on Kv current (Fig. 5a, b). We then tested whether GSSG would also induce a negative shift in the activation curves for Kv channels. We found that G–V curves of the Kv channel were significantly shifted to more negative potentials after GSSG application. V _1/2 in the presence of GSSG was −1.9 ± 2.2 mV (n = 12), and it was not significantly different from that induced by 10 mM H₂O₂ (−1.7 ± 1.8 mV, n = 14). GSSG also had an effect on the time course of activation (n = 8, Fig. 5c). In the presence of GSSG, the time constant of activation decreased, and the change was even larger than that observed with 10 mM H₂O₂. More importantly, when 10 mM H₂O₂ was added to the GSSG-treated cells, we observed no further change in the G–V curve (V _1/2; −1.0 ± 1.6 mV, n = 12) and activation kinetics (Fig. 5c). These data indicate that GSSG mimicked and occluded the effects of H₂O₂, implying that S-glutathionylation appears to occur in the Kv channel during H₂O₂ application leading to the activation of the channel activity.

To confirm that S-glutathionylation mediates the activation of Kv currents by H₂O₂, we examined the effect of glutathione reductase on the action of H₂O₂. Glutathione reductase reduces GSSG to GSH and prevents oxidation of GSH. Since NADPH is an indispensable cofactor for glutathione reductase activity, NADPH (1 mM) was applied to cells together with glutathione reductase. NADPH alone did not block the stimulatory effect of H₂O₂ on Kv currents (Fig. 5e). However, intracellular glutathione reductase completely abolished the stimulatory effect of H₂O₂ on Kv currents (Fig. 5d). Data summarized in Fig. 5f suggest that increased channel activity by H₂O₂ occurs because of direct modification of thiol groups on the Kv channel by GSSG in rat MASMCs.

Redox status determines the response of Kv current to H₂O₂

Recent studies showed that an increase of ROS is linked to hypertension [17]. Most endogenously produced ROS, including H₂O₂, are derived from mitochondrial respiration [23, 36], wherein 1–2 % of consumed oxygen is converted to superoxide radical and then to H₂O₂ [10, 15]. Since H₂O₂ treatment does not induce relaxation but contraction of vascular smooth muscle cells in hypertensive vessels [28], we hypothesized that the increased basal H₂O₂ preoccupied the activation mechanism of Kv channels, thus rendering H₂O₂ treatment ineffective in activating Kv channels. To mimic the endogenously generated H₂O₂, we directly conveyed H₂O₂ into the cytosol by adding H₂O₂ in the patch pipette. The elevated intracellular level of H₂O₂ increased the Kv currents (Fig. 6a). At +40 mV, 0.1 and 10 mM H₂O₂ increased Kv current density up to 45.7 ± 4.8 pA/pF (n = 21, p < 0.01 vs. control pipette) and 43.8 ± 3.7 pA/pF (n = 21, p < 0.01 vs. control pipette), respectively (Fig. 6a). Notably, the concentration–response relationship was shifted to the left, implying that intracellular H₂O₂ is more effective in elevating Kv currents than extracellular H₂O₂. Under this high level of intracellular H₂O₂, the stimulatory effect of bath-applied H₂O₂ was completely abolished (Fig. 6b). Summarized data in Fig. 6c showed that the basal Kv current density under 0.1 mM of intracellular H₂O₂ was 45.7 ± 4.8 pA/pF (n = 21), and it was not enhanced but unaffected (43.9 ± 4.4 pA/pF, p > 0.05) or rather reduced (37.9 ± 4.6 pA/pF, p < 0.01) by subsequent bath application of 0.1 and 10 mM H₂O₂, respectively. These data suggest that high levels of basal H₂O₂ upregulates Kv currents through S-glutathionylation, which may keep acute exposure to H₂O₂ from regulating the Kv channels.

Fig. 6.

Effects of intracellular redox states on the capacity of H₂O₂ to stimulate Kv channels. a Intracellular application of H₂O₂ induced a concentration-dependent increase of Kv currents (filled red circle, n = 21). Compared to bath application (filled gray square, n = 21), the concentration–response relationship was shifted to the left when H₂O₂ was added intracellularly. Current densities of Kv channels were measured at +40 mV. b Effects of 100 μM H₂O₂ in the pipette solution on the action of H₂O₂. Averaged I–V curves before (filled black square) and after bath application of 0.1 mM H₂O₂ (filled red circle) and 10 mM H₂O₂ (filled blue triangle) are shown. Gray line indicates the averaged I–V relationship with control pipette solution (redrawn from Fig. 2a for comparison). Note that Kv currents with pipette solution containing 100 μM H₂O₂ (filled black square) are much larger than those with control pipette solution (gray line). c Comparative data between Kv current densities measured at +40 mV for H₂O₂ effects in control pipette solution (filled gray circle; n = 21) and those in the pipette containing 100 μM H₂O₂ (filled red circle; n = 21)

Discussion

It is still uncertain whether H₂O₂ acts as a vasodilator [55, 80]. There is even less certainty regarding the signal transduction and/or effector mechanism(s) that may be involved in the vascular response to H₂O₂. For example, it may involve the BK_Ca channel [81], K_ATP channel [40], or Kv channel [55]. The present study resolves some of this complexity by providing direct evidence for an effector molecule that can mediate H₂O₂-induced vasodilation through the Kv channel and proposes that S-glutathionylation underlies the stimulatory effect of H₂O₂ on Kv channels. Further experiments demonstrate that under oxidative conditions, Kv channels were not stimulated but rather inhibited by acute exposure to H₂O₂, suggesting that cellular redox status affected S-glutathionylation of the Kv channel and determined the response of the Kv channel to H₂O₂.

In the present study, H₂O₂ relaxed the rat mesenteric artery that was precontracted with an agonist. In contrast, H₂O₂ failed to relax arteries pretreated with DTT and 4-AP; 1 mM TEA and 100 μM BaCl₂ did not affect the H₂O₂ response. The inability of H₂O₂ to relax arteries pretreated with 4-AP suggested that the relaxation response to H₂O₂ involved stimulation of the Kv channel, a mechanism that has also been suggested from patch-clamp/whole-cell studies employing other cell types [12, 54]. Here, we directly measured the effects of H₂O₂ on Kv channels in MASMCs. H₂O₂ increased 4-AP-sensitive Kv currents in a concentration-dependent manner. This was a result of change in the voltage dependence of activation; 10 mM H₂O₂ shifted voltage dependence of 4-AP-sensitive Kv channel conductance by ~7.6 mV (Fig. 3a, b), and the shift was concentration-dependent. The activation kinetics was also accelerated after exposure to H₂O₂ (Fig. 3c, d). It is worthy to note that unlike the role of BK_Ca channel in H₂O₂-induced dilation of coronary arteries and arterioles [5, 81], the BK_Ca channel did not contribute to the dilatory effect of H₂O₂ in rat mesenteric arteries (Fig. 1). This discrepancy may suggest that the regulatory mechanisms underlying vascular tone and the sensitivity of diverse K⁺ channels to H₂O₂ differ among different types of arteries. The 4-AP-sensitive Kv channels are expressed in high density in myocytes derived from rat mesenteric [75] and human pulmonary [26] arteries and are important targets of receptor agonists [3]. BK_Ca channels are the key determinant of coronary arterial tone [37].

Previous studies have proposed a variety of molecular pathways that can be stimulated by H₂O₂. For example, guanylyl cyclase may underlie H₂O₂ relaxation of pulmonary arteries [11], while arachidonic acid (AA) may mediate a vasodilator effect of H₂O₂ in coronary arteries [5]. Recent studies have shown that H₂O₂ induces S-glutathionylation of the channel protein, thereby affecting channel activity [71, 77, 78]. Since the thiol-specific reducing agent DTT blocked the vasodilatory effect of H₂O₂ (Fig. 1), we considered it possible that S-glutathionylation of the Kv channel protein mediates the stimulatory effect of H₂O₂ on Kv currents in the mesenteric artery smooth muscle. We found that addition of H₂O₂ to MASMCs increased S-glutathionylation of the Kv2.1 channel protein dramatically (Fig. 4). It is well known that Kv2.1 expresses a slow-inactivating, TEA-resistant, and 4-AP-sensitive Kv current in rat and human mesenteric arteries [51]. However, we could not exclude a possible glutathionylation of Kv1.2 and Kv1.5 channel proteins since it might not have been detected due to differences in pull-down efficiency in each Kv channel after S-glutathionylation. To confirm the cause–effect relationship, we blocked S-glutathionylation by using GSH reductase. GSH reductase completely abolished the stimulatory effect of H₂O₂. Addition of exogenous GSSG directly stimulated channel activity. Similar to H₂O₂, GSSG alters channel function by speeding up the activation kinetics and shifting the voltage dependence of channel activation to the left. Bath application of H₂O₂ (10 mM), subsequent to maximal GSSG effect, induced no further change in the Kv channel. These results suggest that S-glutathionylation of the Kv channel protein mediates the stimulatory effect of H₂O₂ on the Kv channel and, consequently, the vasodilatory effects in the mesenteric artery. Interestingly, conditions of increased oxidative stress within smooth muscle cells impaired the capacity of exogenous H₂O₂ to stimulate Kv channels (Fig. 6). Not only was the H₂O₂ stimulatory effect much weaker, but also the inhibitory effect of H₂O₂ was unmasked. The molecular mechanism of how H₂O₂ inhibits Kv channel under oxidative condition is not yet known. However, it can be speculated that since S-glutathionylation of the Kv channel persists and the Kv channels are already maximally enhanced, signals such as cyclooxygenase are involved [5]. Taken together, S-glutathionylation of the Kv channel under elevated basal H₂O₂ levels may be involved in the development of the pathology of the hypertensive vessel. This concept is still speculative; therefore, further studies will be required to test this hypothesis.

In the present study, a high concentration of extracellular H₂O₂ is required to regulate K_V channels. This argument holds for neurons. This can be reflected from the fact that, in the hippocampus, the IC₅₀ value for extracellular H₂O₂ to affect postsynaptic potentials was nearly 6 mM [46]. In contrast to extracellular application, a low level of intracellular H₂O₂ is sufficient to elevate the Kv currents (Fig. 6). This difference suggests that either the permeability of the cell membrane to H₂O₂ is low [7, 8, 31] or the rate of H₂O₂ degradation is high near the cell membrane [39]. In addition, the difference in effects possibly indicates that the modulation of thiol groups takes place mainly on the intracellular side of the plasma membrane. This is further supported by the fact that there are no cysteines in the extracellular location of the Kv 2.1 channel. In Kv2.1 channels, 15 cysteines are present: four in a COOH-terminal domain, three in transmembrane core regions (S2 and S6), and the remaining eight in an NH₂-terminal domain (Supplementary Fig. 1). Our electrophysiological data showed that the reaction of Kv2.1 with H₂O₂ or GSSG caused a pronounced increase in channel kinetics and left shift of steady state activation. However, all cysteines of Kv2.1 channels are located outside of S4, a central component of the voltage sensor. Given that the NH₂ terminus has the largest number of cysteines and it has been shown to participate in channel gating [50], one or more cysteines located at the NH₂ terminus might be involved in the effects of H₂O₂ on channel activation. Further studies are required to ascertain this.

Combined with the fact that endothelial cells can produce up to 500 μM H₂O₂ [27] and myoendothelial gap junction can be a pathway of H₂O₂ from the endothelium to the smooth muscle [16], our results suggest that endothelium-derived H₂O₂ can act as a relaxing factor in mammalian arteries. EDHFs are important factors controlling the vascular tone. Sobey [64] suggested that EDHFs play a major role in conditions of high blood pressure, arteriosclerosis, and diabetes by controlling potassium ion channels. The identity of the EDHFs differs depending on the animal species and type of arteries examined [24, 35, 41, 42, 52, 54]. The four major EDHF candidates are an electrical coupling through myoendothelial gap junctions, potassium ions (K⁺), cytochrome P450 metabolites of AA such as epoxyeicosatrienoic acid, and H₂O₂ [7, 31, 42, 76]. A study published in 1991, for the first time, suggested that H₂O₂ was an EDHF [6]: production of H₂O₂ by hyperpolarization of the endothelium, which consequently acts on vascular smooth muscle cells, causing relaxation of blood vessels. Subsequently, several studies have verified the hypothesis that H₂O₂ is an EDHF in animal and human arteries [35, 41, 42, 48, 76]. Although the contribution of EDHFs to vascular tone is not entirely clear, it is generally accepted that nitric oxide (NO) plays a dominant role in controlling the tone of large conduit blood vessels compared to EDHFs, whereas EDHF is more important in small-resistance blood vessels [14]. Consistent with this notion, we found that acetylcholine-induced endothelium-dependent vasodilation was largely inhibited by catalase in small mesenteric arteries. In contrast, acetylcholine-induced endothelium-dependent vasodilation was largely inhibited by an NO synthase blocker, but not by catalase in the aorta (Supplementary Fig. 2).

Posttranslational modifications (PTMs) are important mechanisms regulating ion channel functions. One of the classical PTMs is protein phosphorylation, and a large number of ion channels are regulated by phosphorylation through protein kinase A (PKA), PKC, and other protein kinases [19, 32, 60-62, 79]. A variety of different types of PTMs (e.g., ubiquitylation, SUMOylation, O-glycosylation/O-GlcNAcylation) exist and are discussed elsewhere [9, 13, 53, 56]. Among all these PTMs, redox-mediated PTM is an important category of PTMs that targets the thiol group of cysteine residues. Recently, redox-mediated PTMs are receiving increasing attention, as they are found in both physiological and pathological conditions, including oxidative stress. S-glutathionylation is a major redox-mediated thiol modulation mechanism, involving the addition of a GSH moiety to the protein. Oxidative stress and ROS facilitate S-glutathionylation. Over the past few years, S-glutathionylation has been increasingly observed in many ion channels such as voltage-gated calcium channels, the ryanodine receptor, and K_ATP channels, all of which contribute to critical cellular functions [1, 71, 77, 78]. Our results indicate that the Kv channel protein is significantly glutathionylated after exposure to H₂O₂ (Fig. 4). As delineated above, S-glutathionylation of the Kv channel resulted in an increase in the Kv currents in myocytes (Fig. 5). Alterations in thiol groups on proteins can alter function through structural changes in the channel protein. Since H₂O₂ changed the channel gating properties (Fig. 3), we propose that S-glutathionylation of the Kv2.1 channel protein causes a structural rearrangement of the channel that results in an increase in voltage sensitivity.

In conclusion, H₂O₂ relaxed rat mesenteric arteries by S-glutathionylation-dependent activation of Kv currents under physiological conditions. Our data suggest that S-glutathionylation of the Kv channel protein is, at least in part, an important and novel mechanism of 4-AP-sensitive Kv current activation by H₂O₂. Identifying the mechanisms underlying the vasoactive effects of H₂O₂ should increase our understanding of diseases where oxidative damage has been implicated such as in hypertension, atherosclerosis, and diabetes mellitus.

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Abbreviations

4-AP

4-Aminopyridine

AA

Arachidonic acid

BioGee

Biotinylated glutathione ethyl ester

BK_Ca

Big-conductance Ca²⁺-activated K⁺

DTT

DL-Dithiothreitol

EDHF

Endothelium-derived hyperpolarizing factor

Em

Membrane potential

GSSG

Oxidized form of glutathione

H₂O₂

Hydrogen peroxide

K_ATP

ATP-sensitive K⁺

K_Ca

Ca²⁺-activated K⁺

Kir

Inward rectifier K⁺

Kv

Voltage-gated K⁺

MASMCs

Mesenteric arterial smooth muscle cells

NO

Nitric oxide

NT

Normal Tyrode

PSS

Physiological salt solution

ROS

Reactive oxygen species

TEA

Tetraethylammonium