Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1975 Nov;8(5):551–557. doi: 10.1128/aac.8.5.551

Minimal Inhibitory Concentration of Dapsone for Mycobacterium leprae in Rats

J H Peters *, G R Gordon †, J F Murray Jr †, A H Fieldsteel †, L Levy †
PMCID: PMC429419  PMID: 1108776

Abstract

To define the minimal inhibitory concentration (MIC) of dapsone (DDS) for Mycobacterium leprae in rats, we determined the relationship between dietary and plasma levels of DDS in uninfected male and female Lewis rats. This knowledge was applied to the design of experiments using rats inoculated in the footpads with M. leprae. The MIC for DDS in male and female rats, respectively, was 1.5 to 4.0 ng and 1.8 to 3.0 ng of DDS/ml of plasma, even though the sexes exhibited markedly different concentrations of DDS when receiving the same dietary level of DDS. These values for the MIC of DDS for M. leprae in rats are nearly identical to the previously determined MIC of DDS for M. leprae in mice.

Full text

PDF
551

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Colwell W. T., Chan G., Brown V. H., DeGraw J. I., Peters J. H., Morrison N. E. Potential antileprotic agents. 1. Inhibition of a model mycobacterial system by diaryl sulfones. J Med Chem. 1974 Jan;17(1):142–144. doi: 10.1021/jm00247a031. [DOI] [PubMed] [Google Scholar]
  2. Ellard G. A., Gammon P. T., Rees R. J., Waters M. F. Studies on the determination of the minimal inhibitory concentration of 4,4'-diamino-diphenyl-sulphone (Dapsone, DDS) against Mycobacterium leprae. Lepr Rev. 1971 Jun;42(2):101–117. doi: 10.5935/0305-7518.19710013. [DOI] [PubMed] [Google Scholar]
  3. Feldman W. H. Gerhard Henrik Armauer Hansen. What did he see and when? Int J Lepr. 1965 Jul-Sep;33(3 Suppl):412–416. [PubMed] [Google Scholar]
  4. Fieldsteel A. H., McIntosh A. H. Effect of neonatal thymectomy and antithymocytic serum on susceptibility of rats to mycobacterium leprae infection. Proc Soc Exp Biol Med. 1971 Nov;138(2):408–413. doi: 10.3181/00379727-138-35908. [DOI] [PubMed] [Google Scholar]
  5. Gelber R., Peters J. H., Gordon G. R., Glazko A. J., Levy L. The polymorphic acetylation of dapsone in man. Clin Pharmacol Ther. 1971 Mar-Apr;12(2):225–238. doi: 10.1002/cpt1971122part1225. [DOI] [PubMed] [Google Scholar]
  6. Hilson G. R. Observations on the inoculation of M. leprae in the foot pad of the white rat. Int J Lepr. 1965 Jul-Sep;33(3 Suppl):662–666. [PubMed] [Google Scholar]
  7. Kirchheimer W. F., Storrs E. E., Binford C. H. Attempts to establish the Armadillo (Dasypus novemcinctus linn.) as a model for the study of leprosy. II. Histopathologic and bacteriologic post-mortem findings in lepromatoid leprosy in the Armadillo. Int J Lepr Other Mycobact Dis. 1972 Jul-Sep;40(3):229–242. [PubMed] [Google Scholar]
  8. Levy L., Biggs J. T., Jr, Gordon G. R., Peters J. H. Disposition of the antileprosy drug, dapsone, in the mouse. Proc Soc Exp Biol Med. 1972 Jul;140(3):937–943. doi: 10.3181/00379727-140-36584. [DOI] [PubMed] [Google Scholar]
  9. Murray J. F., Jr, Gordon G. R., Gulledge C. C., Peters J. H. Chromatographic-fluorometric analysis of antileprotic sulfones. J Chromatogr. 1975 Apr 9;107(1):67–72. doi: 10.1016/s0021-9673(00)82748-x. [DOI] [PubMed] [Google Scholar]
  10. Ozawa T., Shepard C. C., Karat A. B. Application of spectrophotofluorometric procedures to some problems in Mycobacterium leprae infections in mice and man treated with dapsone (DDS), diacetyl-DDS (DADDS), and di-formyl-DDS (DFD). Am J Trop Med Hyg. 1971 Mar;20(2):274–281. doi: 10.4269/ajtmh.1971.20.274. [DOI] [PubMed] [Google Scholar]
  11. Pattyn S. R., Verdoolaeghe-Van Loo G. Letter: Sensitivity of Mycobacterium leprae to dapsone, studied in the rat. Int J Lepr Other Mycobact Dis. 1974 Jan-Mar;42(1):72–73. [PubMed] [Google Scholar]
  12. REES R. J. LIMITED MULTIPLICATION OF ACID-FAST BACILLI IN THE FOOT-PADS OF MICE INOCULATED WITH MYCOBACTERIUM LEPRAE. Br J Exp Pathol. 1964 Apr;45:207–218. [PMC free article] [PubMed] [Google Scholar]
  13. Rees R. J., Weddell A. G. Transmission of human leprosy to the mouse and its clinical implications. Trans R Soc Trop Med Hyg. 1970;64(1):31–47. doi: 10.1016/0035-9203(70)90194-x. [DOI] [PubMed] [Google Scholar]
  14. Shepard C. C. A survey of the drugs with activity against M. leprae in mice. Int J Lepr Other Mycobact Dis. 1971 Apr-Jun;39(2):340–348. [PubMed] [Google Scholar]
  15. Shepard C. C. Chemotherapy of leprosy. Annu Rev Pharmacol. 1969;9:37–50. doi: 10.1146/annurev.pa.09.040169.000345. [DOI] [PubMed] [Google Scholar]
  16. Shepard C. C., McRae D. H. A method for counting acid-fast bacteria. Int J Lepr Other Mycobact Dis. 1968 Jan-Mar;36(1):78–82. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES