Abstract
Nonviable, 5-iodouracil (IUra)-substituted bacteriophage T4td8 can be multiplicity reactivated. The data indicate that two nonviable, IUra-substituted T4td8 phage can complement each other intracellularly to produce viable progeny. Phage particles in lysates of T4td8-infected Escherichia coli BT−, prepared in the presence of varying mole fractions of IUra plus thymine, were examined by infecting with low and high dilutions of lysate. The yields of multiplicity reactivable particles were identical, regardless of the mole fractions of IUra present in the growth media. However, the yields of viable phage, measured at low multiplicities of infection, decreased with increasing mole fraction of IUra. The results are consistent with the hypothesis that the lethal effect of IUra is a consequence of its incorporation into DNA. Further, the IUra-induced lesion cannot involve genetic damage that shuts off expression at a single region of the genome.
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Selected References
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- DUNN D. B., SMITH J. D. Effects of 5-halogenated uracils on the growth of Escherichia coli and their incorporation into deoxyribonucleic acids. Biochem J. 1957 Nov;67(3):494–506. doi: 10.1042/bj0670494. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freese E., Freese E. B. Mutagenic and inactivating DNA alterations. Radiat Res. 1966;(Suppl):97+–97+. [PubMed] [Google Scholar]
- Goz B., Prusoff W. H. The ability of phage containing 5-iodo-2'-deoxyuridine-subsituted deoxyribonucleic acid to induce enzymes. J Biol Chem. 1968 Sep 25;243(18):4750–4756. [PubMed] [Google Scholar]
- HARM W. On the mechanism of multiplicity reactivation in bacteriophage. Virology. 1956 Aug;2(4):559–564. doi: 10.1016/0042-6822(56)90011-3. [DOI] [PubMed] [Google Scholar]
- HERSHEY A. D., CHASE M. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol. 1952 May;36(1):39–56. doi: 10.1085/jgp.36.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LITMAN R. M., PARDEE A. B. Production of bacteriophage mutants by a disturbance of deoxyribonucleic acid metabolism. Nature. 1956 Sep 8;178(4532):529–531. doi: 10.1038/178529b0. [DOI] [PubMed] [Google Scholar]
- Luria S. E. Reactivation of Irradiated Bacteriophage by Transfer of Self-Reproducing Units. Proc Natl Acad Sci U S A. 1947 Sep;33(9):253–264. doi: 10.1073/pnas.33.9.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PRUSOFF W. H., BAKHLE Y. S., MCCREA J. F. INCORPORATION OF 5-IODO-2'-DEOXYURIDINE INTO THE DEOXYRIBONUCLEIC ACID OF VACCINIA VIRUS. Nature. 1963 Sep 28;199:1310–1311. doi: 10.1038/1991310a0. [DOI] [PubMed] [Google Scholar]
- STREISINGER G., EDGAR R. S., DENHARDT G. H. CHROMOSOME STRUCTURE IN PHAGE T4. I. CIRCULARITY OF THE LINKAGE MAP. Proc Natl Acad Sci U S A. 1964 May;51:775–779. doi: 10.1073/pnas.51.5.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sartorelli A. C., Fischer D. S., Downs W. G. Use of sarcoma 180/TG to prepare hyperimmune ascitic fluid in the mouse. J Immunol. 1966 Apr;96(4):676–682. [PubMed] [Google Scholar]
- Sharp D. G. Multiplicity reactivation of animal viruses. Prog Med Virol. 1968;10:64–109. [PubMed] [Google Scholar]
- Snustad D. P. Dominance interactions in Escherichia coli cells mixedly infected with bacteriophage T4D wild-type and amber mutants and their possible implications as to type of gene-product function: catalytic vs. stoichiometric. Virology. 1968 Aug;35(4):550–563. doi: 10.1016/0042-6822(68)90285-7. [DOI] [PubMed] [Google Scholar]