Abstract
Ribonucleic acid (RNA) synthesis in the sorbitol-dependent, fragile yeast mutant VY1160 (Venkov et al., 1974) is rapidly inhibited by rifampin. The growth of the mutant cells and protein synthesis are more slowly affected by the antibiotic, apparently as secondary phenomena. Lower doses of rifampin (50 to 100 μg/ml) preferentially inhibit ribosomal RNA synthesis in comparison to that of messenger RNA and transfer RNA. Transcription and translation of messenger RNA continues in the presence of low doses of rifampin, as evidenced by the unimpaired induction of α-glucosidase. Partially purified RNA polymerase II from this mutant, in contrast to that from the parental strain, is strongly inhibited by low concentrations (1 μg/ml) of rifampin, whereas RNA polymerase I from the two strains is similar in behavior. The mutant may be useful for the study of regulatory mechanisms of transcription in eukaryotes.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adman R., Schultz L. D., Hall B. D. Transcription in yeast: separation and properties of multiple FNA polymerases. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1702–1706. doi: 10.1073/pnas.69.7.1702. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Busiello E., Di Girolamo A., Di Girolamo M., Fischer-Fantuzzi L., Vesco C. Multiple effects of rifamycin derivatives on animal-cell metabolism of macromolecules. Eur J Biochem. 1973 Jun;35(2):251–258. doi: 10.1111/j.1432-1033.1973.tb02832.x. [DOI] [PubMed] [Google Scholar]
- Hartwell L. H. Macromolecule synthesis in temperature-sensitive mutants of yeast. J Bacteriol. 1967 May;93(5):1662–1670. doi: 10.1128/jb.93.5.1662-1670.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Medoff G., Kobayashi G. S., Kwan C. N., Schlessinger D., Venkov P. Potentiation of rifampicin and 5-fluorocytosine as antifungal antibiotics by amphotericin B (yeast-membrane permeability-ribosomal RNA-eukaryotic cell-synergism). Proc Natl Acad Sci U S A. 1972 Jan;69(1):196–199. doi: 10.1073/pnas.69.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meilhac M., Tysper Z., Chambon P. Animal DNA-dependent RNA polymerases. 4. Studies on inhibition by rifamycin derivatives. Eur J Biochem. 1972 Jul 13;28(2):291–300. doi: 10.1111/j.1432-1033.1972.tb01913.x. [DOI] [PubMed] [Google Scholar]
- Riva S., Silvestri L. G. Rifamycins: a general view. Annu Rev Microbiol. 1972;26:199–224. doi: 10.1146/annurev.mi.26.100172.001215. [DOI] [PubMed] [Google Scholar]
- Van Wijk R., Ouwehand J., van den Bos T., Koningsberger V. V. Induction and catabolite repression of alpha-glucosidase synthesis in protoplasts of Saccharomyces carlsbergensis. Biochim Biophys Acta. 1969 Jul 22;186(1):178–191. doi: 10.1016/0005-2787(69)90501-2. [DOI] [PubMed] [Google Scholar]
- Venkov P. V., Hadjiolov A. A., Battaner E., Schlessinger D. Saccharomyces cerevisiae: sorbitol-dependent fragile mutants. Biochem Biophys Res Commun. 1974 Feb 4;56(3):599–604. doi: 10.1016/0006-291x(74)90646-9. [DOI] [PubMed] [Google Scholar]
- Wintersberger E., Wintersberger U. Rifamycin insensitivity of RNA synthesis in yeast. FEBS Lett. 1970 Jan 15;6(1):58–60. doi: 10.1016/0014-5793(70)80043-6. [DOI] [PubMed] [Google Scholar]
- Wintersberger E., Wintersberger U. Rifamycin insensitivity of RNA synthesis in yeast. FEBS Lett. 1970 Jan 15;6(1):58–60. doi: 10.1016/0014-5793(70)80043-6. [DOI] [PubMed] [Google Scholar]