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. 1976 Jan;9(1):42–48. doi: 10.1128/aac.9.1.42

Inhibition of Sporulation by Cerulenin and Its Reversion by Exogenous Fatty Acids in Saccharomyces cerevisiae

Tadao Ohno a,1, Juichi Awaya a, Satoshi Ōmura a
PMCID: PMC429471  PMID: 769672

Abstract

Sporulation of Saccharomyces cerevisiae G2-2 was inhibited by the antibiotic cerulenin which is known to be a specific inhibitor of fatty acid and sterol synthesis. This inhibition was reversed by various fatty acids, especially by oleic acid (C18:1) and pentadecanoic acid (C15:0). Ergosterol showed only slight reversibility of this inhibition. When cerulenin was added to the sporulation medium later than 12 h after the start of incubation, the marked inhibition disappeared. When the fatty acid fraction extracted from the sporulated yeasts was added to the cells pretreated with cerulenin for more than 6 h, sporulation became evident 6 h after the fatty acid fraction addition. Therefore, sufficient synthesis of fatty acids required for sporulation was assumed to occur during the first 6 h in phase I of yeast sporulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. D'Agnolo G., Rosenfeld I. S., Awaya J., Omura S., Vagelos P. R. Inhibition of fatty acid synthesis by the antibiotic cerulenin. Specific inactivation of beta-ketoacyl-acyl carrier protein synthetase. Biochim Biophys Acta. 1973 Nov 29;326(2):155–156. doi: 10.1016/0005-2760(73)90241-5. [DOI] [PubMed] [Google Scholar]
  2. Esposito M. S., Esposito R. E., Arnaud M., Halvorson H. O. Acetate utilization and macromolecular synthesis during sporulation of yeast. J Bacteriol. 1969 Oct;100(1):180–186. doi: 10.1128/jb.100.1.180-186.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  4. Fowell R. R. Factors controlling the sporulation of yeasts. II. The sporulation phase. J Appl Bacteriol. 1967 Dec;30(3):450–474. doi: 10.1111/j.1365-2672.1967.tb00323.x. [DOI] [PubMed] [Google Scholar]
  5. Goldberg I., Walker J. R., Bloch K. Inhibition of lipid synthesis in Escherichia coli cells by the antibiotic cerulenin. Antimicrob Agents Chemother. 1973 May;3(5):549–554. doi: 10.1128/aac.3.5.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HASHIMOTO T., GERHARDT P., CONTI S. F., NAYLOR H. B. Studies on the fine structure of microorganisms. V. Morphogenesis of nuclear and membrane structures during ascospore formation in yeast. J Biophys Biochem Cytol. 1960 Apr;7:305–310. doi: 10.1083/jcb.7.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haber J. E., Halvorson H. O. Regulation of sporulation in yeast. Curr Top Dev Biol. 1972;7:61–83. doi: 10.1016/s0070-2153(08)60069-1. [DOI] [PubMed] [Google Scholar]
  8. Henry S. A., Halvorson H. O. Lipid synthesis during sporulation of Saccharomyces cerevisiae. J Bacteriol. 1973 Jun;114(3):1158–1163. doi: 10.1128/jb.114.3.1158-1163.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. ITAYA K., UI M. COLORIMETRIC DETERMINATION OF FREE FATTY ACIDS IN BIOLOGICAL FLUIDS. J Lipid Res. 1965 Jan;6:16–20. [PubMed] [Google Scholar]
  10. Illingworth R. F., Rose A. H., Beckett A. Changes in the lipid composition and fine structure of Saccharomyces cerevisiae during ascus formation. J Bacteriol. 1973 Jan;113(1):373–386. doi: 10.1128/jb.113.1.373-386.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kawaguchi A. Control of ergosterol biosynthesis in yeast. Existence of lipid inhibitors. J Biochem. 1970 Feb;67(2):219–227. doi: 10.1093/oxfordjournals.jbchem.a129245. [DOI] [PubMed] [Google Scholar]
  12. MUNDKUR B. Electron microscopical studies of frozendried yeast. III. Formation of the tetrad in Saccharomyces. Exp Cell Res. 1961 Oct;25:24–40. doi: 10.1016/0014-4827(61)90304-4. [DOI] [PubMed] [Google Scholar]
  13. Ohno T., Awaya J., Kesado T., Nomura S., Omura S. Mechanism of action of CM-55, a synthetic analogue of the antilipogenic antibiotic cerulenin. Antimicrob Agents Chemother. 1974 Oct;6(4):387–392. doi: 10.1128/aac.6.4.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ono T., Kesado T., Awaya J., Omura S. Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast. Biochem Biophys Res Commun. 1974 Apr 23;57(4):1119–1124. doi: 10.1016/0006-291x(74)90812-2. [DOI] [PubMed] [Google Scholar]
  15. Roth R., Halvorson H. O. Sporulation of yeast harvested during logarithmic growth. J Bacteriol. 1969 May;98(2):831–832. doi: 10.1128/jb.98.2.831-832.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schweizer E., Bolling H. A Saccharomyces cerevisiae mutant defective in saturated fatty acid biosynthesis. Proc Natl Acad Sci U S A. 1970 Oct;67(2):660–666. doi: 10.1073/pnas.67.2.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vance D., Goldberg I., Mitsuhashi O., Bloch K. Inhibition of fatty acid synthetases by the antibiotic cerulenin. Biochem Biophys Res Commun. 1972 Aug 7;48(3):649–656. doi: 10.1016/0006-291x(72)90397-x. [DOI] [PubMed] [Google Scholar]

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