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. 1976 Jan;9(1):42–48. doi: 10.1128/aac.9.1.42

Inhibition of Sporulation by Cerulenin and Its Reversion by Exogenous Fatty Acids in Saccharomyces cerevisiae

Tadao Ohno a,1, Juichi Awaya a, Satoshi Ōmura a
PMCID: PMC429471  PMID: 769672

Abstract

Sporulation of Saccharomyces cerevisiae G2-2 was inhibited by the antibiotic cerulenin which is known to be a specific inhibitor of fatty acid and sterol synthesis. This inhibition was reversed by various fatty acids, especially by oleic acid (C18:1) and pentadecanoic acid (C15:0). Ergosterol showed only slight reversibility of this inhibition. When cerulenin was added to the sporulation medium later than 12 h after the start of incubation, the marked inhibition disappeared. When the fatty acid fraction extracted from the sporulated yeasts was added to the cells pretreated with cerulenin for more than 6 h, sporulation became evident 6 h after the fatty acid fraction addition. Therefore, sufficient synthesis of fatty acids required for sporulation was assumed to occur during the first 6 h in phase I of yeast sporulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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