Abstract
Several N-substituted erythromycylamines were evaluated for their ability to inhibit the binding of [14C]erythromycin to ribosomes. The association and dissociation constants for the binding of each compound to Escherichia coli ribosomes were determined. These studies have resulted in the development of three types of probes for topological studies of the erythromycin-binding site and the ribosome: the chemically reactive bromoacetamido, the photoreactive N-(2)-nitro-4-azidophenyl)glycinamido, and the fluorescent fluorescein isothiocyanate derivatives of 9(S)-erythromycylamine.
Full text
PDF





Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Harris R., Pestka S. Studies on the formation of transfer ribonucleic acid-ribosome complexes. XXIV. Effects of antibiotics on binding of aminoacyl-oligonucleotides to ribosomes. J Biol Chem. 1973 Feb 25;248(4):1168–1174. [PubMed] [Google Scholar]
- Massey E. H., Kitchell B. S., Martin L. D., Gerzon K. Antibacterial activity of 9(S)-erythromycylamine-aldehyde condensation products. J Med Chem. 1974 Jan;17(1):105–107. doi: 10.1021/jm00247a018. [DOI] [PubMed] [Google Scholar]
- Massey E. H., Kitchell B., Martin L. D., Gerzon K., Murphy H. W. Erythromycylamine. Tetrahedron Lett. 1970 Jan;(2):157–160. doi: 10.1016/s0040-4039(01)97664-6. [DOI] [PubMed] [Google Scholar]
- Pestka S. Binding of [14C]erythromycin to Escherichia coli ribosomes. Antimicrob Agents Chemother. 1974 Oct;6(4):474–478. doi: 10.1128/aac.6.4.474. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pestka S. Inhibitors of ribosome functions. Annu Rev Microbiol. 1971;25:487–562. doi: 10.1146/annurev.mi.25.100171.002415. [DOI] [PubMed] [Google Scholar]
- Pestka S., Lemahieu R. A. Effect of erythromycin analogues on binding of [14C]erythromycin to Escherichia coli ribosomes. Antimicrob Agents Chemother. 1974 Oct;6(4):479–488. doi: 10.1128/aac.6.4.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pestka S., Lemahieu R., Miller P. Correlation of effects of erythromycin analogues on intact bacteria and on [14C]erythromycin binding to Escherichia coli ribosomes. Antimicrob Agents Chemother. 1974 Oct;6(4):489–491. doi: 10.1128/aac.6.4.489. [DOI] [PMC free article] [PubMed] [Google Scholar]